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B3279

Sigma-Aldrich

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

>98% (TLC)

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Synonym(s):
L-BAPA, L-BAPNA, BANI
Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
21653-40-7
Molecular Weight:
434.88
Beilstein:
4081878
EC Number:
244-505-6
MDL number:
MFCD00063682
UNSPSC Code:
12352204
PubChem Substance ID:
24891697
NACRES:
NA.32

Quality Level

200

Assay

>98% (TLC)

form

powder

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

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General description

Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride (L-BAPNA) is a colorless, chromogenic substrate for proteolytic enzymes such as trypsin, actinidin, and papain.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as substrate:
  • to measure papain activity in spectrophotometric amidase assay
  • to measure the inhibitory function of human cystatin C (HCC) on the protease activity of papain by colorimetric assay
  • for trypsin

Substrates

Chromogenic substrate for trypsin and other proteolytic enzymes.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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