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Key Documents

Safety Information

B2661

Sigma-Aldrich

Anti-fd Bacteriophage−Biotin antibody produced in rabbit

~3.5 mg/mL, IgG fraction of antiserum

Synonym(s):

Anti-fd Bacteriophage

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

biotin conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

concentration

~3.5 mg/mL

technique(s)

indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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Specificity

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Immunogen

fd bacteriophage

Application

Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000100745
0000100745
0000088047
018M4884
077M4896

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. The description states Product B2661, Anti-fd Bacteriophage, may be used for staining DNA, chromosomes and nuclei, and for fluorescence microscopy or flow cytometry.  Are there other uses for the product?

    The product has also been used for the detection of apoptosis, applied as a label to live cells, as a component of BrdU cell cycle investigation.

  4. What is the expected solution stability for aqueous solutions of Product B2661, Anti-fd Bacteriophage?

    Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.

  5. Which is more cell permeable, H33342 or H33258?

    H33342 has been found to be about 10 times more cell-permeable than H33258. See Science, 282(5387), 296-298, (1998).

  6. What is the specificity for H33342?

    The bisBenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators.  Reference: Loontiens, F. G., et al., Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)] and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities. Biochemistry, 29, p. 9029 (1990).

  7. When using Product B2661, Anti-fd Bacteriophage, which is the preferred Hoechst stain for chromosomes?

    Hoechst No. 33258 and not Hoechst No. 33342 is the preferable dye for chromosomal staining.

  8. What is the pH of Product B2661, Anti-fd Bacteriophage, in solution?

    There is no pH test specification for product B2261 on a lot-to-lot basis.  A one-time measurement of the pH of a 2% solution was 1.9. It has been observed that this material will precipitate from phosphate buffer solutions.

  9. Will chromosomes that are dividing or replicating stain with Product B2661, Anti-fd Bacteriophage?

    No; chromosomes that are dividing or replicating will not stain with this dye.

  10. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  11. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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