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B1656

Sigma-Aldrich

Monoclonal Anti-Rat Kappa & Lambda Light Chains−Biotin antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Rat Kappa & Lambda Light Chains

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About This Item

UNSPSC Code:
12352203

biological source

mouse

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RT-39/RL-6, monoclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa

species reactivity

rat

should not react with

mouse, human, horse, rabbit, goat, sheep, cat, chicken, pig, dog

technique(s)

direct ELISA: 1:40,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:600
western blot (chemiluminescent): 1:40,000

isotype

IgG1/IgG2a

shipped in

dry ice

storage temp.

−20°C

General description

Binds all rat IgG1/IgG2a
Mixture from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.
The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.
The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Application

Monoclonal Anti-Rat Kappa & Lambda Light Chains-Biotin antibody produced in mouse may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a working antibody dilution of 1:600. A dilution of 1:4000 is recommended for dot blot. A working antibody dilution of 1:40000 may be used for ELISA and immunoblotting.

Biochem/physiol Actions

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either kappa or the lambda chain. The B cells express one type of light chain during their life time. The coupling of biotin to monoclonal Anti-Rat Kappa & Lambda Light Chains antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Lot/Batch Number
019H4874
010K4813
021K4898
011K4879

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Jerry A Katzmann et al.
Clinical chemistry, 48(9), 1437-1444 (2002-08-27)
The detection of monoclonal free light chains (FLCs) is an important diagnostic aid for a variety of monoclonal gammopathies and is especially important in light-chain diseases, such as light-chain myeloma, primary systemic amyloidosis, and light-chain-deposition disease. These diseases are more

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