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B1125

Sigma-Aldrich

Bathocuproinedisulfonic acid disodium salt

7.2-9.1% purity dry basis (ICP), powder

Synonym(s):

2,9-Dimethyl-4,7-diphenyl-1,10-phenantrolinedisulfonic acid disodium salt, Disodium bathocuproinedisulfonate

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About This Item

Empirical Formula (Hill Notation):
C26H18N2Na2O6S2
CAS Number:
52698-84-7
Molecular Weight:
564.54
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Bathocuproinedisulfonic acid disodium salt, for spectrophotometric det. of Cu, Fe

Assay

7.2-9.1% dry basis (ICP)

Quality Level

200

form

powder

quality

for spectrophotometric det. of Cu, Fe

technique(s)

titration: 90% using HCLO4 (Dry Basis)

color

white to beige

solubility

H2O: 50 mg/mL

suitability

suitable for determination of copper

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H20N2O6S2.2Na/c1-15-25(35(29,30)31)21(17-9-5-3-6-10-17)19-13-14-20-22(18-11-7-4-8-12-18)26(36(32,33)34)16(2)28-24(20)23(19)27-15;;/h3-14H,1-2H3,(H,29,30,31)(H,32,33,34);;/q;2*+1/p-2

InChI key

RNGKZLRAVYPLJC-UHFFFAOYSA-L

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General description

Bathocuproinedisulfonic acid is a strong copper chelator. It is used for the determination of copper and iron in biological samples.

Application

  • Bathocuproinedisulfonic acid disodium salt has been used as a copper chelator for specific assay in Arabidopsis seedlings.
  • It has been used as an electron chain-associated free radical production inhibitor for superoxide dismutase assay in cells.
  • It has been added to the culture media of trypanosomes as a copper chelator for the stabilization of cysteine.

Other Notes

Mixture of isomers

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tomoo Shirabe et al.
Analytical chemistry, 80(23), 9360-9362 (2009-06-25)
A novel method has been devised for the determination of phytochelatins (PCs), heavy-metal-tolerant peptides produced by higher plants and algae. The method is based on the facts that fluorescence of bathocuproine disulfonate (BCS) is quenched by Cu(I) ions as a
Kristine Hardy et al.
Free radical biology & medicine, 37(10), 1550-1563 (2004-10-13)
Antioxidants can inhibit the proliferation of T lymphocytes induced by mitogens. This has been postulated to be due to their scavenging of reactive oxygen species which may act as second messengers in the antigen-induced signaling cascade leading to cell proliferation.
Vicente Sancenón et al.
The Journal of biological chemistry, 279(15), 15348-15355 (2004-01-17)
Copper plays a dual role in aerobic organisms, as both an essential and a potentially toxic element. To ensure copper availability while avoiding its toxic effects, organisms have developed complex homeostatic networks to control copper uptake, distribution, and utilization. In
Simple procedure for the single sample determination of serum copper and iron.
B ZAK
Clinica chimica acta; international journal of clinical chemistry, 3(4), 328-334 (1958-07-01)
Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue.
Weydert CJ and Cullen JJ
Nature Protocols, 5, 51-66 (2010)
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