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AL11430

Sigma-Aldrich

DMT-locA(bz) Phosphoramidite

configured for ABI

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Empirical Formula (Hill Notation):
C48H52N7O8P
CAS Number:
206055-79-0
Molecular Weight:
885.94
UNSPSC Code:
41116105
NACRES:
NA.51

biological source

non-animal source (BSE/TSE no Risk)

Quality Level

300

product line

Proligo Reagents

Assay

≥98.0% (31P-NMR)
≥98.0% (reversed phase HPLC)

form

powder

impurities

≤3 wt. % Residual Solvent Content
<0.4% Water Content (Karl Fischer)
<0.5% Single unspecified Impurity (reversed phase HPLC)

color

white to off-white

solubility

soluble, clear, colorless

absorption

<0.1 in acetonitrile at 0.2 M

suitability

conforms to structure for H-NMR
conforms to structure for LC-MS

compatibility

configured for ABI

storage temp.

−20°C

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General description

DMT-locA(bz) Phosphoramidite belongs to the group of LNA phosphoramidite. It is known as DMT-locAdenosine(N6-Benzoyl)- ß-Cyanoethylphosphoramidite. The synthesis cycle for Locked Nucleic Acid (LNA) oligonucleotides consists of the same series of reactions as the cycle that is employed for DNA monomers. However, the rate of coupling for LNA monomers is slower compared to that of DNA monomers (a coupling time of 8 minutes is recommended for LNA monomers compared to 90 seconds for DNA monomers).

With the exception of the LNA monomers, LNA synthesis is accomplished with the same reagents as DNA synthesis. LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran (THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.

Features and Benefits

Key Features of LNA Phosphoramidites :

  • LNA oligonucleotides are prepared by phosphoramidite chemistry
  • Standard DNA synthesizer platforms can be employed. No change is required in the reagents commonly used for DNA synthesis
  • To further enhance the hybridization characteristics of LNA, 5-methyl-cytidine is employed instead of cytidine
  • LNA monomers are as soluble in acetonitrile as their DNA counterparts (except for the 5-methyl-cytidine derivative, which requires the application of 10-20%, dichloromethane as a co-solvent)
  • Mixmer oligonucleotides containing LNA, DNA and/or RNA monomers can be assembled easily
  • LNA oligonucleotides with predefined melting temperatures (Tm) can be designed and prepared

Other Notes

LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran(THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.

Legal Information

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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