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AEC101

Sigma-Aldrich

AEC Staining Kit

liquid

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Synonym(s):
ACE substrate kit
UNSPSC Code:
41116134
NACRES:
NA.32

form

liquid

Quality Level

200

usage

sufficient for ≥1,000 tests (150 ml substrate solution)

storage temp.

2-8°C

Related Categories

Application

AEC Staining Kit has been used as a substrate
  • for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods
  • for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay
  • for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads

Packaging

Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.

Caution

Stable at least one year at 2-8 °C.

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Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

WGK

WGK 3

Flash Point(F)

134.6 °F

Flash Point(C)

57 °C

Regulatory Information

危险化学品

Certificates of Analysis (COA)

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Vincenti D, et al.
Molecular Medicine, 9(3), 105-105 (2003)
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Tang T, et al.
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Kaiyuan Ni et al.
Chem, 5(7), 1892-1913 (2019-08-07)
Checkpoint blockade immunotherapy (CBI) elicits durable therapeutic responses by blocking T cell inhibitory pathways of tumors with pre-infiltrated T cells and/or high mutational burden to activate antitumor immunity but is ineffective against poorly immunogenic tumors. Immunogenic radiotherapy, photodynamic therapy (PDT)
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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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