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Sigma-Aldrich

Alkaline Phosphatase Blue Membrane Substrate Solution

alkaline phosphatase substrate, solution

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About This Item

UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.83

product name

Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate

form

solution

Quality Level

200

usage

sufficient for 400 mL working substrate

storage temp.

2-8°C

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General description

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

Application

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
  • to analyse direct alkaline phosphatase (AP) activity
  • to perform alkaline phosphatase assays with colorimetric system
  • to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Generation of a human iPSC line from a patient with Leigh syndrome
Galera T, et al.
Stem Cell Research, 16(1), 63-66 (2016)
Inhibition of alkaline phosphatase: an emerging new drug target
Al-Rashida M and Iqbal J
Mini-Reviews in Medicinal Chemistry, 15(1), 41-51 (2015)
The Short Isoform of NF-YA Belongs to the Embryonic Stem Cell Transcription Factor Circuitry
Dolfini D, et al.
Stem Cells, 30(11), 2450-2459 (2012)
Giulia Coppiello et al.
Stem cell research, 21, 40-43 (2017-07-06)
We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector. Cells present a normal karyotype and
Victoria Cerrada et al.
Stem cell research, 40, 101566-101566 (2019-09-12)
Human iPSC line, IISHDOi006-A, was obtained from fibroblasts of a patient with Dominant Optic Atrophy (DOA) carrying a heterozygous mutation in the gene ACO2: c.1999G>A; p.Glu667Lys. Reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc were delivered using a non-integrative methodology that
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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