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A9799

Sigma-Aldrich

Agar

High gel strength, suitable for plant cell culture

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
NACRES:
NA.21

biological source

algae (Rhodophyceae)

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

transition temp

transition temp 32-38 °C (1.5%)

gel strength

>1000 g/cm2

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar is a natural polymer extracted from red alga Rhodophyceae, primarily, consisting of alternative D- and L-galactose units. This type of high gel strength agar is used when a firmer gel is required and mostly suitable for plant cell culture.
Agar is a polysaccharide complex obtained through bleaching and hot water extraction of agarocytes from the red alga Rhodophyceae, found in the Pacific and Indian Oceans and the sea of Japan. The genera Gelidium, Acanthopeltis, Ceramium, Pterocladia and Gracilaria predominate in agar production. Agar is composed of about 70% agarose and 30% agaropectin.

Application

Agar has been used:
  • as a component of the fruit fly diet
  • as a component of saline-agar for the approximation of gray matter from brain
  • in in vitro root nodulation assay of Glycine max seeds

Agar has been used:
  • in the preparation of laboratory diet to maintain the culture of D. melanogaster wild-type larva
  • as a high gel strength agar in the seed culture media to grow Arabidopsis thaliana mutant seeds
  • to construct a tissue-mimicking phantom to test the imaging performance of an ultrasound transducer

Biochem/physiol Actions

Agar with a higher percentage of anhydrous units in the backbone of their chemical composition provides superior mechanical stiffness and strength to the pristine hydrogel.

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Agar gel strength: A correlation study between chemical composition and rheological properties
Bertasa M, et al.,
European Polymer Journal, 123, 109442-109442 (2020)
Takaaki Ogura et al.
Frontiers in plant science, 11, 563-563 (2020-06-26)
Magnesium (Mg) is essential for many biological processes in plant cells, and its deficiency causes yield reduction in crop systems. Low Mg status reportedly affects photosynthesis, sucrose partitioning and biomass allocation. However, earlier physiological responses to Mg deficiency are scarcely
Shannon M Boucousis et al.
NeuroImage, 63(3), 1237-1248 (2012-08-21)
Integrating intracranial EEG (iEEG) with functional MRI (iEEG-fMRI) may help elucidate mechanisms underlying the generation of seizures. However, the introduction of iEEG electrodes in the MR environment has inherent risk and data quality implications that require consideration prior to clinical
Improved nodulation and seed yield of soybean (Glycine max) with a new isoflavone-based inoculant of Bradyrhizobium elkanii
Riviezzi B, et al.
Rhizosphere, 15, 100219-100219 (2020)
Lindsey J Gray et al.
Journal of insect physiology, 104, 60-70 (2017-12-06)
Optimal life-history strategies are those that best allocate finite environmental resources to competing traits. We used the geometric framework for nutrition to evaluate life-history strategies followed by Drosophila melanogaster by measuring the condition-dependent performance of life-history traits, including the morphology
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