A9655

Auramine O

Name: Auramine O
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Empirical Formula (Hill Notation):

C₁₇H₂₁N₃ · HCl

CAS Number:

2465-27-2

Molecular Weight:

303.83

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24891484

EC Number:

219-567-2

MDL number:

MFCD00012484

Colour Index Number:

41000

Beilstein/REAXYS Number:

4030061

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Product Name

Auramine O, Dye content ≥80 %, certified by the BSC

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

agency

certified by the BSC

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

yellow to green

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λ_max

370 nm in H₂O, 432 nm in H₂O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Hematology Stains

Histology Stains

Tissue Diagnostics

General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

pictograms

GHS08,GHS07,GHS09

signalword

Warning

hcodes

H302,H319,H351,H411

pcodes

P201 - P273 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

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Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).

Inbaraj B S, et al.

Biochemical Engineering Journal, 31(3), 204-215 (2006)

E Kohen.

Cell Structure and Function by Microspectrofluorometry, 490-490 (2014)

E W Koneman.

Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)

Lynne S.

Practical Guide to Diagnostic Parasitology, 486-486 (2009)

Auramine-O as a fluorescence marker for the detection of amyloid fibrils.

Nadav Amdursky et al.

The journal of physical chemistry. B, 116(45), 13389-13395 (2012-10-24)

There is an indispensable need for a fluorescence marker for the detection of amyloid fibrils, where, at present, the most used marker is thioflavin-T (ThT). Here, we present the use of auramine-O (AuO) as a possible alternative to ThT. As

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Auramine O