A9325
L-Alanine 4-nitroanilide hydrochloride
protease substrate, ≥99% (TLC), powder
Synonym(s):
Ala-4-nitroanilide, H-Ala-pNA
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About This Item
Empirical Formula (Hill Notation):
C9H11N3O3 · HCl
CAS Number:
Molecular Weight:
245.66
NACRES:
NA.83
PubChem Substance ID:
eCl@ss:
32160406
UNSPSC Code:
12352204
EC Number:
250-811-0
MDL number:
Beilstein/REAXYS Number:
6303731
Product Name
L-Alanine 4-nitroanilide hydrochloride, protease substrate
InChI
1S/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H/t6-;/m0./s1
InChI key
YEXRLSXNWLNHQR-RGMNGODLSA-N
SMILES string
Cl[H].C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O
assay
≥99% (TLC)
form
powder
solubility
water: 50 mg/mL, clear to very slightly hazy
storage temp.
−20°C
Quality Level
Application
L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum).
Biochem/physiol Actions
L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Methods for Measuring Urinary Enzyme Activities
Urinary Enzymes, 99-145 (1992)
Effect of insoluble fiber supplementation applied at different ages on digestive organ weight and digestive enzymes of layer-strain poultry
Yokhana J S, et al.
Poultry Science, 95(3), 550-559 (2015)
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