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A9325

Sigma-Aldrich

L-Alanine 4-nitroanilide hydrochloride

protease substrate, ≥99% (TLC), powder

Synonym(s):

Ala-4-nitroanilide, H-Ala-pNA

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About This Item

Empirical Formula (Hill Notation):
C9H11N3O3 · HCl
CAS Number:
Molecular Weight:
245.66
Beilstein:
6303731
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.83

product name

L-Alanine 4-nitroanilide hydrochloride, protease substrate

Quality Level

Assay

≥99% (TLC)

form

powder

solubility

water: 50 mg/mL, clear to very slightly hazy

storage temp.

−20°C

SMILES string

Cl[H].C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H/t6-;/m0./s1

InChI key

YEXRLSXNWLNHQR-RGMNGODLSA-N

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Application

L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum).

Biochem/physiol Actions

L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effect of insoluble fiber supplementation applied at different ages on digestive organ weight and digestive enzymes of layer-strain poultry
Yokhana J S, et al.
Poultry Science, 95(3), 550-559 (2015)
Methods for Measuring Urinary Enzyme Activities
Urinary Enzymes, 99-145 (1992)

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