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Merck
CN

A9275

Avidin from egg white

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

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About This Item

CAS Number:
UNSPSC Code:
12352203
EC Number:
215-783-6
NACRES:
NA.46
MDL number:
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Product Name

Avidin from egg white, BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

biological source

egg white

product line

BioUltra

form

lyophilized powder

specific activity

≥10 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa
subunit 16 kDa

storage temp.

2-8°C

Quality Level

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Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies. The product has also been used as a blocking agent in ELISA applications.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Avidin protein is a homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Other Notes

One unit will bind 1.0 μg of d-biotin.

Preparation Note

Affinity purified

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物源性产品
低风险生物材料
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Roland Hager et al.
Scientific reports, 7, 42075-42075 (2017-02-16)
Arrays of individual molecules can combine the advantages of microarrays and single-molecule studies. They miniaturize assays to reduce sample and reagent consumption and increase throughput, and additionally uncover static and dynamic heterogeneity usually masked in molecular ensembles. However, realizing single-DNA
Lee B Sims et al.
Journal of nanobiotechnology, 15(1), 67-67 (2017-10-07)
Advanced stage cancer treatments are often invasive and painful-typically comprised of surgery, chemotherapy, and/or radiation treatment. Low transport efficiency during systemic chemotherapy may require high chemotherapeutic doses to effectively target cancerous tissue, resulting in systemic toxicity. Nanotherapeutic platforms have been
Roland Hager et al.
Small (Weinheim an der Bergstrasse, Germany), 12(21), 2877-2884 (2016-04-12)
The biofunctionalization of nanopatterned surfaces with DNA origami nanostructures is an important topic in nanobiotechnology. An unexplored challenge is, however, to co-immobilize proteins with DNA origami at pre-determined substrate sites in high contrast relative to the nontarget areas. The immobilization
Q Wang et al.
The American journal of physiology, 275(5 Pt 2), R1584-R1592 (1998-10-29)
We examined the effect of endotoxemia in mice on protein and mRNA levels for the acute phase proteins complement C3 and serum amyloid A (SAA) in jejunal mucosa. Endotoxemia was induced in mice by the subcutaneous injection of 250 microg
B Steiniger et al.
The American journal of pathology, 159(2), 501-512 (2001-08-04)
We investigate the white pulp compartments of 73 human spleens and demonstrate that there are several microanatomical peculiarities in man that do not occur in rats or mice. Humans lack a marginal sinus separating the marginal zone (MZ) from the

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