A9171
Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Rabbit Anti-Chicken IgY (IgG) (whole molecule)−AP
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About This Item
biological source
rabbit
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:35,000
dot blot: 1:100,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): suitable
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Immunoglobulin Y (IgY) is also known as egg yolk immunoglobulin and is found mainly in birds, amphibians and reptiles. IgY cannot activate mammalian complement system and cannot bind to mammalian rheumatoid factors, and protein A or G. IgY facilitates an essential role in veterinary science, functional food and bio-products. It also has a crucial role in controlling infectious diseases of bacterial and viral origin.
Specificity
Anti-Chicken IgY (IgG) (whole molecule)-Alkaline Phosphatase antibody is specific for chicken IgY (IgG).
Immunogen
IgY (IgG) isolated from pooled normal chicken serum
IgY (IgG) isolated from pooled normal chicken serum.
Application
Anti-Chicken IgY (IgG) (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in:
- immunoblotting
- indirect enzyme linked immunosorbent assay (ELISA)
- immunoglobulin assay
- dot Blot
- immunohistology
Biochem/physiol Actions
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Paulina Escandon et al.
Veterinary medicine (Auckland, N.Z.), 10, 141-150 (2019-12-10)
This study evaluated the specificity of different avian secondary antibodies used in Western blot and dot-blot ELISA to detect avian bornavirus antibodies in bird plasma. Plasma samples were collected from: two Blue and gold macaws, one positive and one negative
Yu-Chun Cai et al.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 27(6), 527-530 (2010-03-18)
Egg yolk immunoglobulin (IgY) is a major immunoglobulin from birds, amphibians and reptiles. IgY does not activate mammalian complement system, and does not bind to mammalian rheumatoid factors, protein A or G. In addition, it has a high yield, and
Effect of trypsin inhibitor activity in soya bean on growth performance, protein digestibility and incidence of sub-clinical necrotic enteritis in broiler chicken flocks
Palliyeguru MW, et al.
British Poultry Science, 52(3), 359-367 (2011)
M W C D Palliyeguru et al.
British poultry science, 52(3), 359-367 (2011-07-08)
1. The effect of three different levels of dietary trypsin inhibitor activity (achieved by varying the amount of non-toasted full fat soya bean in replacement for toasted full fat soya bean) on the incidence of spontaneously-occurring sub-clinical necrotic enteritis (NE)
Effect of dietary protein concentrates on the incidence of subclinical necrotic enteritis and growth performance of broiler chickens
Palliyeguru MWCD
Poultry Science, 89(1), 34-43 (2010)
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