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A9046

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti Chicken Igy
MDL number:
MFCD00162225
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

chicken

technique(s)

direct ELISA: 1:30,000
dot blot: 1:160,000 (indirect chemiluminescence)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.

Immunogen

purified chicken IgG

Application

Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA), dierct ELISA, dot blot and immunohistochemistry.
Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications.

Biochem/physiol Actions

Egg yolk IgY immunoglobulin acquired from hen have been studied extensively. It is useful in immunological assays and does interact with the rheumatoid factor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Will Product A9046, Anti-Chicken IgY, also recognize chicken IgG?

    This antibody (and possibly other anti-chicken antibodies) recognizes both chicken IgG from serum and chicken IgY from egg yolk, because these immunoglobulins are structurally very similar.

  6. How is chicken IgY different from chicken IgG?

    Chicken IgG refers to antibodies from the chicken serum and chicken IgY antibodies are from the yolk.

  7. Product A9046, Anti-Chicken IgY, is peroxidase conjugated.  Is this similar to horseradish peroxidase?

    The peroxidase used to make this product is indeed peroxidase from horseradish (HRP).

  8. Do we have additional anti-chicken IgY conjugates?

    We provide anti-chicken IgY antibodies that are conjugated to alkaline phosphatase (Product No. A9171) and FITC (Product No. F8888).  We also provide the unconjugated anti-chicken IgY antibody (Product No. C2288).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Antal Nyeste et al.
The Journal of biological chemistry, 291(9), 4473-4486 (2016-01-02)
The prion protein (PrP) seems to exert both neuroprotective and neurotoxic activities. The toxic activities are associated with the C-terminal globular parts in the absence of the flexible N terminus, specifically the hydrophobic domain (HD) or the central region (CR).
Aline Sardinha-Silva et al.
PLoS pathogens, 15(6), e1007871-e1007871 (2019-06-22)
Infection of host cells by Toxoplasma gondii is an active process, which is regulated by secretion of microneme (MICs) and rhoptry proteins (ROPs and RONs) from specialized organelles in the apical pole of the parasite. MIC1, MIC4 and MIC6 assemble
Production and purification of IgY antibodies from chicken egg yolk.
Amro W A, et al.
Journal of Genetic Engineering and Biotechnology, 16(1), 99-103 (2018)
Frequency of anti-Toxocara antibodies in broiler chickens in southern Brazil
Oliveira A, et al,
Revista Brasileira de Parasitologia veterinaria = Brazilian Journal of Veterinary Parasitology : Org?o Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 27(2), 141-145 (2018)
Germline polymorphisms and survival of lung adenocarcinoma patients: A genome-wide study in two European patient series
Galvan A, et al.
International Journal of Cancer. Journal International Du Cancer, 136(5), E262-E271 (2015)
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