A8957
Adenosine-5′-triphosphate Sulfurylase from Saccharomyces cerevisiae
lyophilized powder, ≥1.0 units/mg protein
Synonym(s):
ATP:Sulfate adenylyltransferase
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100 MG
CN¥1,606.86
CN¥1,606.86
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100 MG
CN¥1,606.86
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
CN¥1,606.86
In StockDetails
Recommended Products
form
lyophilized powder
specific activity
≥1.0 units/mg protein
composition
Protein, ≥40% modified Warburg-Christian
foreign activity
hexokinase ≤5%
storage temp.
−20°C
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Application
Adenosine-5′-triphosphate sulfurylase (ATP sulfurylase) may be used to study sulfur metabolism and hydrogen sulfide biosynthesis. ATP sulfurylase is also used in pyrosequencing.[1] The enzyme has been used along with luciferase in a bioluminescent assay for inorganic sulfate quantitation in freshwater. Inorganic sulfate is converted, by ATP sulfurylase, into adenosine-5′-phosphosulfate and inorganic pyrophosphate, with consumption of ATP. The remaining ATP is used as a co-factor in the reaction catalyzed by firefly luciferase, generating photons of visible light as a co-product. The light output is inversely proportional to the content of inorganic sulfate.[2]
Biochem/physiol Actions
Adenosine-5′-triphosphate sulfurylase catalyzes the first intracellular reaction of sulfate assimilation, which forms adenosine-5′-phosphosulfate (APS) from inorganic sulfate and ATP. It is involved in sulfate activation and the biosynthesis pathway of sulfur-containing amino acids. The expression of ATP sulfurylase is dependent on the formation of the MET4-MET28-MET31 and MET4-MET28-MET32 complexes on its 5′ upstream region [3].
Unit Definition
One unit will produce 1.0 μmole of ATP from APS and inorganic pyrophosphate per min at pH 8.0 at 30°C.
Physical form
Contains citrate buffer salts
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Haiping Wu et al.
Analytical chemistry, 83(9), 3600-3605 (2011-03-29)
In pyrosequencing chemistry, four cascade enzymatic reactions with the catalysis of polymerase, adenosine triphosphate (ATP) sulfurylase, luciferase, and apyrase are employed. The sensitivity of pyrosequencing mainly depends on the concentration of luciferase which catalyzes a photoemission reaction. However, the side-reaction
An optimized bioluminescent assay for inorganic sulfate quantitation in freshwater
Marques SM, et al.
Analytical Methods : Advancing Methods and Applications, 5, 1317-1327 (2013)
Olga A Gandelman et al.
PloS one, 5(11), e14155-e14155 (2010-12-15)
The real-time monitoring of polynucleotide amplification is at the core of most molecular assays. This conventionally relies on fluorescent detection of the amplicon produced, requiring complex and costly hardware, often restricting it to specialised laboratories. Here we report the first
Ali Agah et al.
Nucleic acids research, 32(21), e166-e166 (2004-12-04)
Pyrosequencing is a DNA sequencing technique based on sequencing-by-synthesis enabling rapid real-time sequence determination. This technique employs four enzymatic reactions in a single tube to monitor DNA synthesis. Nucleotides are added iteratively to the reaction and in case of incorporation
Cintia G Kawashima et al.
The Plant journal : for cell and molecular biology, 66(5), 863-876 (2011-03-16)
MicroRNAs play a key role in the control of plant development and response to adverse environmental conditions. For example, microRNA395 (miR395), which targets three out of four isoforms of ATP sulfurylase, the first enzyme of sulfate assimilation, as well as
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