A8957
Adenosine-5′-triphosphate Sulfurylase from Saccharomyces cerevisiae
lyophilized powder, ≥1.0 units/mg protein
Synonym(s):
ATP:Sulfate adenylyltransferase
form
lyophilized powder
specific activity
≥1.0 units/mg protein
composition
Protein, ≥40% modified Warburg-Christian
foreign activity
hexokinase ≤5%
storage temp.
−20°C
Application
Adenosine-5′-triphosphate sulfurylase (ATP sulfurylase) may be used to study sulfur metabolism and hydrogen sulfide biosynthesis. ATP sulfurylase is also used in pyrosequencing. The enzyme has been used along with luciferase in a bioluminescent assay for inorganic sulfate quantitation in freshwater. Inorganic sulfate is converted, by ATP sulfurylase, into adenosine-5′-phosphosulfate and inorganic pyrophosphate, with consumption of ATP. The remaining ATP is used as a co-factor in the reaction catalyzed by firefly luciferase, generating photons of visible light as a co-product. The light output is inversely proportional to the content of inorganic sulfate.
Biochem/physiol Actions
Adenosine-5′-triphosphate sulfurylase catalyzes the first intracellular reaction of sulfate assimilation, which forms adenosine-5′-phosphosulfate (APS) from inorganic sulfate and ATP. It is involved in sulfate activation and the biosynthesis pathway of sulfur-containing amino acids. The expression of ATP sulfurylase is dependent on the formation of the MET4-MET28-MET31 and MET4-MET28-MET32 complexes on its 5′ upstream region .
Physical form
Contains citrate buffer salts
Other Notes
One unit will produce 1.0 μmole of ATP from APS and inorganic pyrophosphate per min at pH 8.0 at 30°C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Haiping Wu et al.
Analytical chemistry, 83(9), 3600-3605 (2011-03-29)
In pyrosequencing chemistry, four cascade enzymatic reactions with the catalysis of polymerase, adenosine triphosphate (ATP) sulfurylase, luciferase, and apyrase are employed. The sensitivity of pyrosequencing mainly depends on the concentration of luciferase which catalyzes a photoemission reaction. However, the side-reaction
An optimized bioluminescent assay for inorganic sulfate quantitation in freshwater
Marques SM, et al.
Analytical Methods : Advancing Methods and Applications, 5, 1317-1327 (2013)
Isabelle Boutet et al.
BMC genomics, 12, 530-530 (2011-11-01)
The deep-sea hydrothermal vent mussel Bathymodiolus azoricus harbors thiotrophic and methanotrophic symbiotic bacteria in its gills. While the symbiotic relationship between this hydrothermal mussel and these chemoautotrophic bacteria has been described, the molecular processes involved in the cross-talking between symbionts
Ali Agah et al.
Nucleic acids research, 32(21), e166-e166 (2004-12-04)
Pyrosequencing is a DNA sequencing technique based on sequencing-by-synthesis enabling rapid real-time sequence determination. This technique employs four enzymatic reactions in a single tube to monitor DNA synthesis. Nucleotides are added iteratively to the reaction and in case of incorporation
Olga A Gandelman et al.
PloS one, 5(11), e14155-e14155 (2010-12-15)
The real-time monitoring of polynucleotide amplification is at the core of most molecular assays. This conventionally relies on fluorescent detection of the amplicon produced, requiring complex and costly hardware, often restricting it to specialised laboratories. Here we report the first
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