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Merck
CN

A8580

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

Synonym(s):

Anti-Glutathione-S-Transferase, Anti-GST

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit, IgG fraction of antiserum, PBS suspension

biological source

rabbit

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS suspension

mol wt

antigen 27.5 kDa

should not react with

porcine, bovine, human, rabbit, rat

technique(s)

ELISA: suitable
immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GSTP1(2950)

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Application

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.
Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Biochem/physiol Actions

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
048M4775V
025M4812V
052M4825
031M4863
029K4799

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Clinical pathology in non-clinical toxicology testing
Haschek and Rousseaux's Handbook of Toxicologic Pathology, 112(47), 565-594 (2013)
Metabolism of xenobiotics of human environments
Progress in Molecular Biology and Translational Science, 112, 31-88 (2012)
Xun Shang et al.
The Journal of biological chemistry, 282(12), 8801-8811 (2007-02-03)
p200 RhoGAP, a member of the Rho GTPase-activating protein (RhoGAP) family, was previously implicated in the regulation of neurite outgrowth through its RhoGAP activity. Here we show that ectopic expression of p200 RhoGAP stimulates fibroblast cell proliferation and cell cycle
Jessica Gagnon et al.
Epigenetics, 10(8), 677-691 (2015-06-16)
O-GlcNAcylation is a posttranslational modification catalyzed by the O-Linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) and reversed by O-GlcNAcase (OGA). Numerous transcriptional regulators, including chromatin modifying enzymes, transcription factors, and co-factors, are targeted by O-GlcNAcylation, indicating that this modification is central for
The role of glutathione-S-transferase in anti-cancer drug resistance
Townsend D M and Tew K D
Oncogene, 22(47), 7369-7369 (2003)
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