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A8549

Sigma-Aldrich

Albumin, biotin labeled bovine

lyophilized powder

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Synonym(s):
Biotin labeled albumin
CAS Number:
9048-46-8
MDL number:
MFCD00130395
UNSPSC Code:
12352202
eCl@ss:
34058011
NACRES:
NA.25

biological source

bovine

Quality Level

200

Assay

≥80% protein basis (biuret)

form

lyophilized powder

extent of labeling

8-16 mol biotin per mol albumin

technique(s)

bioconjugation: suitable

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Related Categories

Application

Biotin labeled bovine albumin has been used as a component in myosin V processivity experiments. It has also been used to provide biotin tags for neutravidin coating and also to suppress nonspecific cell adhesion during preparation of tension gauge tether (TGT) surface.
Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues. In spleen, biotin-BSA binds to red pulp sinuses, but not white pulp vessels (including the central arteriole) nor the marginal sinus. In the thymus, prominent binding was restricted to the capillaries of the corticomedullary area.

Physical form

Contains sodium citrate buffer salts

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Product A8549, Albumin, biotin labeled bovine, soluble in?

    This product is soluble in water. This product contains sodium citrate buffer salts, so addition of a buffer is probably not necessary; just adding water is sufficient.

  4. What is the solution stability of Product A8549, Albumin, biotin labeled bovine?

    Sigma Aldrich has not tested the stability of this product in solution.  It is recommended to prepare fresh solutions.

  5. What is the linker between the BSA and the biotin for Product A8549, Albumin, biotin labeled bovine?

    There is no linker or "spacer". The link between the BSA and the biotin is an amide bond connecting an amino group on the BSA to the carboxy group on the biotin.

  6. What is the method of preparation for Product A8549, Albumin, biotin labeled bovine?

    The method of preparation of this product is considered proprietary by Sigma-Aldrich.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Do you have the amino acid sequence for bovine serum albumin?

    Bovine Serum Albumin is produced as a protein of 607 amino acids. The signal peptide (amino acids 1-18) and the propeptide (amino acids 19-22) are cleaved to make the mature form.  The mature form of BSA is 583 amino acids (amino acids 25-607, Uniprot P02769).  The sequence for the mature protein is below:DTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLTSSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA

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