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Merck
CN

A8481

Anti-γ-Actin (ACTG1) Antibody

mouse monoclonal, 2-2.1.14.17

Synonym(s):

Anti-ACTG1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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Product Name

Anti-γ-Actin antibody, Mouse monoclonal, clone 2-2.1.14.17, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2-2.1.14.17, monoclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

mouse, chicken, canine, bovine, human, hamster

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells

isotype

IgG1

UniProt accession no.

P63261

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... ACTG1(404122)
chicken ... ACTG1(415296)
human ... ACTG1(71)
mouse ... Actg1(11465)

Related Categories

General description

γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.
Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH2-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.

Application

Anti-γ-Actin antibody, Mouse monoclonal has been used in :
  • immunofluorescence
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
  • immunohistochemistry

Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.
Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.

Biochem/physiol Actions

Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000258939
0000258939
0000196933
0000137583
0000137583

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Jianguo Sun et al.
OncoTargets and therapy, 12, 3019-3030 (2019-05-23)
Background: Cancer metastasis is the leading cause of cancer-related death in breast cancer. However, our understanding of its mechanisms is still limited. At this study, the biological roles and clinical significance of NRF3 (NFE2L3, nuclear factor, Erythroid 2 Like 3)
V V Philimonenko et al.
Histochemistry and cell biology, 141(3), 229-239 (2014-01-23)
Simultaneous detection of biological molecules by means of indirect immunolabeling provides valuable information about their localization in cellular compartments and their possible interactions in macromolecular complexes. While fluorescent microscopy allows for simultaneous detection of multiple antigens, the sensitive electron microscopy
Actin, Myosin, and Cell Movement
The Cell: A Molecular Approach (2000)
Cell damage-induced conformational changes of the pro-apoptotic protein Bak in vivo precede the onset of apoptosis.
Griffiths, GJ.
Cell, 144, 903-914 (1999)
Emily E Stackpole et al.
Biology open, 8(8) (2019-08-23)
RNA-binding proteins (RBPs) function in higher-order assemblages such as RNA granules to regulate RNA localization and translation. The Fragile X homolog FXR2P is an RBP essential for formation of neuronal Fragile X granules that associate with axonal mRNA and ribosomes
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