A8481
Anti-γ-Actin antibody, Mouse monoclonal
clone 2-2.1.14.17, purified from hybridoma cell culture
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All Photos(4)
Synonym(s):
Anti-ACTG1
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
2-2.1.14.17, monoclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
mouse, chicken, canine, bovine, human, hamster
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
bovine ... ACTG1(404122)
chicken ... ACTG1(415296)
human ... ACTG1(71)
mouse ... Actg1(11465)
Related Categories
General description
γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.
Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH2-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.
Specificity
Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.
Application
Anti-γ-Actin antibody, Mouse monoclonal has been used in :
- immunofluorescence
- enzyme linked immunosorbent assay (ELISA)
- western blotting
- immunohistochemistry
Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.
Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.
Biochem/physiol Actions
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Emily E Stackpole et al.
Biology open, 8(8) (2019-08-23)
RNA-binding proteins (RBPs) function in higher-order assemblages such as RNA granules to regulate RNA localization and translation. The Fragile X homolog FXR2P is an RBP essential for formation of neuronal Fragile X granules that associate with axonal mRNA and ribosomes
Actin, Myosin, and Cell Movement
The Cell: A Molecular Approach (2000)
Cell damage-induced conformational changes of the pro-apoptotic protein Bak in vivo precede the onset of apoptosis.
Griffiths, GJ.
Cell, 144, 903-914 (1999)
Actin post-translational modifications: the cinderella of cytoskeletal control
Varland S, et al.
Trends in Biochemical Sciences (2019)
Guobin Jiang et al.
Molecular medicine reports, 18(6), 5417-5426 (2018-10-27)
Increasingly, evidence has revealed that aberrant microRNA (miRNA) expression is involved in breast cancer carcinogenesis and further progression, including metastasis. miRNA (miR)‑27a was previously identified to be abnormally expressed and to serve pro‑oncogenic functions in multiple human cancer types, including
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