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A7921

Sigma-Aldrich

Agar

suitable for plant cell culture, powder

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.21

biological source

algae (Rhodophyceae)

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Application

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
  • of Gamborg′s B5 regeneration medium
  • of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
  • in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Other Notes

Purified for research and protoplast culture.

Preparation Note

Typical working concentration: 6-12 g/L.

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocol: a method to study the direct reprogramming of lateral root primordia to fertile shoots
Kareem A, et al.
Plant methods, 12(1), 27-27 (2016)
Thidiazuron induced micropropagation of Hypericum triquetrifolium Turra
Oluk, E and Orhan, Serpil
African Journal of Biotechnology, 8(15), 771-777 (2009)
AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus
Rabaiolli SMS, et al.
CERNE, 23(2), 185-192 (2017)
Ata Chizari et al.
Biomedical optics express, 11(5), 2352-2365 (2020-06-06)
Functional performance of handheld laser speckle contrast imaging (LSCI) is compromised by movement artefacts. Here we quantify the movements of a handheld LSCI system employing electromagnetic (EM) tracking and measure the applied translational, tilt and on-surface laser beam speeds. By
Bety Shiue de Hsie et al.
Journal of photochemistry and photobiology. B, Biology, 198, 111577-111577 (2019-08-04)
Lippia rotundifolia Cham. is in the family Verbenaceae and is endemic to the Cerrado. This species is aromatic and characterized by the presence of glandular trichomes on its leaves that are rich in monoterpenes. The objective of this study was
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