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A7851

Sigma-Aldrich

Monoclonal Anti-Hamster IgG−Peroxidase antibody produced in mouse

clone MAH 1.12, purified immunoglobulin, lyophilized powder

Synonym(s):

Monoclonal Anti-Hamster IgG

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

MAH 1.12, monoclonal

form

lyophilized powder

species reactivity

hamster

should not react with

rat, bovine, horse, chicken, goat, human, mouse, pig, rabbit

technique(s)

direct ELISA: 1:40,000 using hamster IgG as the coating protein
western blot: 1:2,000 using recombinant mouse TNFα

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgGs are glycoprotein antibodies that modulate several immune responses. Hamster IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-hamster IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Mouse Monoclonal Anti-hamster IgG-Peroxidase antibody is immunospecific for hamster IgG and does not recognize human, bovine, horse, goat, pig, rabbit, rat, mouse, and chicken IgG.

Immunogen

purified Armenian hamster monoclonals (TN3-19.2 and H22).

Application

Mouse Monoclonal Anti-Hamster IgG-Peroxidase antibody is suitable for ELISA (1:40,000) and western blotting (1:2,000) applications.
Parasite cell lysates were analyzed by western blot using goat anti-hamster IgG at a 1:5000 dilution for 1 hour at room temperature.

Physical form

Lyophilized powder containing 0.01 M phosphate buffered saline, pH 7.4, 1% bovine serum albumin, and 0.01% merthiolate.

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
027M4751
085M4831
013M4794
129K4846
037K4838

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