A7107
Monoclonal Anti-AP2 antibody produced in mouse
~1.0 mg/mL, clone A6/2/2, purified immunoglobulin, buffered aqueous solution
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Anti-AP2TF, Anti-TFAP2
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
A6/2/2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: suitable
western blot: 2-4 μg/mL using G361 total cell extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TFAP2A(7020)
General description
Adaptor protein-2 (AP2) is a key constituent of clathrin-coated vesicles that mediates the endocytosis of cell membrane components such as G protein-coupled receptors (GPCRs). AP2 is a heterotetramer that consists of α, β, μ and σ subunits which bind to tyrosine- and dileucine-based motifs on membrane-associated cargo proteins.
Monoclonal Anti-AP2 (mouse IgG1 isotype) is derived from the hybridoma A6/2/2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to amino acids of human AP2α. In humans and mice, the adaptor protein-2 (AP2) family comprises five different transcription factors namely AP2α, AP2β, AP2 γ, AP2δ, and AP2ε. These proteins contain N-terminal transactivation domain and C-terminal helix-span-helix motif, which together with a central basic region facilitates dimerization and DNA binding.
Immunogen
peptide corresponding to amino acid 415-433 of human AP2α.
Application
Monoclonal Anti-AP2 antibody produced in mouse has been used in:
- immunofluorescence staining
- immunoblotting
- immunohistochemistry
Biochem/physiol Actions
Adaptor protein-2 (AP2) expression is associated with the embryonic development. AP2β was found to be a tumor specific human telomerase reverse transcriptase (hTERT) promoter activator, suggesting it may be a biomarker for cancer diagnosis or as a cancer therapeutic target for inhibiting hTERT activity and tumor cell growth. In humans, mutations or loss of these genes result in increased tumor growth and metastasis. Specifically, AP2α loss causes down regulation of E-cadherin and matrix metallopeptidase 9 (MMP-9) activity, which in turn enhance tumorigenicity of colon cancer cells. This effect may also be the result of AP2α regulation by p53.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Roles of telomeres and telomerase in cancer, and advances in telomerase-targeted therapies
Genome Medicine, 8(1), 69-69 (2016)
Loss of AP-2alpha results in deregulation of E-cadherin and MMP-9 and an increase in tumorigenicity of colon cancer cells in vivo.
Oncogene, 26(28), 4049-4049 (2007)
Tumor-specific activation of human telomerase reverses transcriptase promoter activity by activating enhancer-binding protein-2$\beta$ in human lung cancer cells
The Journal of biological chemistry, 282(36), 26460-26470 (2007)
AP-2alpha and AP-2gamma are transcriptional targets of p53 in human breast carcinoma cells
Oncogene, 25(39), 5405-5405 (2006)
Short mitochondrial ARF triggers Parkin/PINK1-dependent mitophagy.
The Journal of Biological Chemistry, 289(43), 29519-29530 (2014)
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