A7058
Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal
clone HIS-1, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
UNSPSC Code:
12352203
NACRES:
NA.56
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biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Specificity
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody suitable for immunoblotting. Working dilution 1:20
Also suitable for dot blot assays and ELISA
Also suitable for dot blot assays and ELISA
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Reconstitution
The antibody conjugate should be reconstituted with 0.5 ml deionized water.
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
Hazard Classifications
Skin Sens. 1
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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