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A7054

Sigma-Aldrich

ACCUSPIN™ System-Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

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MDL number:
MFCD01092091
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

400

sterility

aseptically filled

form

solution

shelf life

Expiry date on the label.

packaging

pkg of package 12 tubes

density

1.077 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

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General description

ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 50 mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). Each tube is aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.

Application

ACCUSPIN System-Histopaque-1077 is used for the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradient separation media. Histopaque-1077 has been used for:

  • human lymphocyte antigen (HLA) typing
  • initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes.
  • preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
  • isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)citation
  • isolation of human lymphocytescitation
  • isolation of breast cancer circulating tumor cells (CTCs) from the whole bloodcitation
ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

Features and Benefits

ACCUSPIN™ System-Histopaque®-1077 offers numerous advantages, such as:
  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Histopaque is sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, and resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

Quantity

Package contains 12 prefilled tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What are the most commonly used anticoagulants for the Accuspin tubes (Product A7054)?

    EDTA or heparin are the most commonly used anticoagulants.  Heparin however should not be used when performing RNA assays.  EDTA can interfere with certain calcium binding assays.  Other anticoagulants have been reported to give acceptable results.

  4. Does peripheral blood need to be diluted prior to loading into the Accuspin tubes (Product A7054)?

    No.  The blood may either be used without dilution or with a 1:2 dilution.   In house studies at Sigma Aldrich have showed no decrease in recovery for peripheral blood specimens loaded directly into the Accuspin tubes.

  5. May buffy coats or donor packs with reduced red cell numbers be used with Accuspin tubes (Product A7054)?

    No.  For the mononuclear cells to band above the "frit", the specimen must contain a sufficient number of red cells to displace the density gradient above the "frit".

  6. Can granulocytes be collected using the Accuspin tubes (Product A7054)?

    No.  After centrifugation, the granulocytes will end up at the bottom of the Accuspin tube - just above the packed red blood cells.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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