Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

A6982

Sigma-Aldrich

HydroMatrix™ Peptide Cell Culture Scaffold

mixture, powder

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207

biological source

synthetic

Quality Level

100

form

powder

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

HydroMatrix has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.

Components

HydroMatrix is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from fluid precursors into highly crosslinked peptide hydrogels when presented with increased temperatures or ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the HydroMatrix solution, researchers can control the flexibility of the 3-D architecture to meet their application′s specific needs.

Caution

The lyophilized product at -20°C and is stable for many years at this temperature. The hydrogel should be stored at -70°C to maximize its active span. The 1% (w/v) stock solution should be stored -20°C and is stable for 3-6 months.

Preparation Note

This product is supplied as a lyophilized powder. A 1% (w/v) stock solution at 10 mg/mL can be prepared with addition of sterile water, resulting in a rigid gel. A 0.5% (w/v) solution will lead to a softer gel that is more suitable for cell migration and angiogenesis. Lower concentrations in the range of 0.15%-0.25% are more suitable for many cell types including neurons. In aqueous solution, HydroMatrix has a pH of 2.5 and should not be added directly to cells without prior addition of a buffer or medium. Once the hydrogel has formed, do not attempt to mix the gel any further, as this risks destroying the hydrogel integrity. Manipulation of the cells should be carefully performed to avoid disruption of the scaffold.

Legal Information

HydroMatrix is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What volume of H2O should be used for reconstitution of Product A6982, HydroMatrix™ Peptide Cell Culture Scaffold?

    The package size of the A6982 indicates the volume of H2O to use for reconstitution.  For example, a 1 ml package will require 1 ml of  H2O for reconstitution.

  6. Will Product A6982, HydroMatrix™ Peptide Cell Culture Scaffold, work will for adherent cells and suspension cells?   

    Yes, the product works with both cell types.

  7. What is the concentration of Product A6982, HydroMatrix™ Peptide Cell Culture Scaffold, when reconstituted as indicated?

    A 1% (w/v) (10 mg/ml) stock solution is accomplished by the addition of sterile dH20 as indicated.

  8. What concentration of HydroMatrix™ Peptide Scaffold, Product A6982, should I use for my cells?

    A 1% (w/v) solution results in a stronger gel. A working concentration of 0.5% (w/v) results in a soft gel with weak mechanical strength that is suitable for cell migration and angiogenesis.  Working solution concentrations as low as 0.15-0.25% are suitable for many cell types, including neurons. It is recommended for most applications to start with a 0.25% (w/v) working solution (2.5 mg/ml).

  9. How do I embed or encapsulate cells in HydroMatrix™ Peptide Scaffold, Product A6982, before polymerization?

    In a solution of dH20, HydroMatrix™ has a pH of 2.0-2.5; therefore it must not be added directly to cells.  The addition of buffer or media is performed prior to the addition of cells.  If performing cell encapsulation studies, addition of iso-osmotic sucrose will help protect the cells until a physiological pH is obtained.

  10. How does HydroMatrix™ Peptide Cell Culture Scaffold compare to PuraMatrix from Becton-Dickenson?

    Both products are peptide hydrogels.  There is no similarity between the peptides used to create the matrices.  They are both just classified as self-assembling peptide hydrogels. The sequence for the peptide is considered proprietary.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Daniela Stoppoloni et al.
Rheumatology international, 33(9), 2399-2403 (2012-03-28)
Osteoarthritis (OA) is one of the most common degenerative joint disease for which there is no cure. It is treated mainly with non-steroidal anti-inflammatory drugs to control the symptoms and some supplements, such as glucosamine and chondroitin sulphate in order
Amish Asthana et al.
Drug discovery today, 18(11-12), 533-540 (2012-12-29)
Force and substrate physical property (pliability) is one of three well established microenvironmental factors (MEFs) that may contribute to the formation of physiologically more relevant constructs (or not) for cell-based high-throughput screening (HTS) in preclinical drug discovery. In 3D cultures
Amish Asthana et al.
Drug discovery today, 17(15-16), 810-817 (2012-04-10)
The three microenvironmental factors that characterize 3D cultures include: first, chemical and/or biochemical composition, second, spatial and temporal dimensions, and third, force and/or substrate physical properties. Although these factors have been studied individually, their interdependence and synergistic interactions have not
Josine E G Vaes et al.
Glia, 69(3), 655-680 (2020-10-13)
Encephalopathy of prematurity (EoP) is a common cause of long-term neurodevelopmental morbidity in extreme preterm infants. Diffuse white matter injury (dWMI) is currently the most commonly observed form of EoP. Impaired maturation of oligodendrocytes (OLs) is the main underlying pathophysiological

Articles

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service