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A6778

Sigma-Aldrich

Angiotensin Converting Enzyme from rabbit lung

≥2.0 units/mg protein (modified Warburg-Christian)

Synonym(s):

ACE, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase

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About This Item

CAS Number:
9015-82-1
Enzyme Commission number:
3.4.15.1 (BRENDA, IUBMB)
MDL number:
MFCD00130325
UNSPSC Code:
12352204
eCl@ss:
42010109
NACRES:
NA.32

form

lyophilized powder

Quality Level

200

specific activity

≥2.0 units/mg protein (modified Warburg-Christian)

shipped in

dry ice

storage temp.

−20°C

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General description

The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site. ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure. Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril. Currently, developing inhibitory peptides from natural food sources, or phenolic compounds from plant sources to inhibit ACE is underway. ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.

Application

Angiotensin converting enzyme from rabbit lung has been used:
  • for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)
  • to measure the ACE inhibition by litchi pericarp and cooked chicken breast using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC respectively
  • in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.

Biochem/physiol Actions

Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.

Quality

May contain traces of sodium chloride.

Unit Definition

One unit will produce 1.0 μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.

inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are there any other known inhibitors of rabbit lung Angiotensin Converting Enzyme (ACE), Product A6778?

    Idrapril, the prototype of a new class of angiotensin converting enzyme (ACE) inhibitors, competitively inhibits, with nanomolar apparent Ki, the hydrolysis of hippuryl-glycyl-glycine by rabbit lung ACE.  Kinetic Ki and kon and koff values were 470 pM, 3.0±1.5×106 M-1sec-1 and 1.4±0.3×10-3 sec-1, respectively.  Reference: Biochemical Pharmacology, 45(6), 1358-1362 (1993).

  6. What is the medical significance of Angiotensin Converting Enzyme (ACE), Product A6778?

    Angiotensin-converting enzyme (ACE) produces angiotensin II, a substance that affects the cardiovascular system in numerous ways, including narrowing of blood vessels. This narrowing can cause high blood pressure and cause the heart to work harder.  ACE inhibitors treat a variety of conditions, such as high blood pressure, scleroderma and migraines. (Mayo Clinic)

  7. What is the effect of bleomycin on Angiotensin Converting Enzyme (ACE) activity in vivo?

    Bleomycin causes a marked reduction in rabbit lung ACE activity. Reference: Cancer Research, 40, 3621-3626, (1980).

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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