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A6659

Sigma-Aldrich

AMPSO

≥99% (titration)

Synonym(s):

N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C7H17NO5S
CAS Number:
68399-79-1
Molecular Weight:
227.28
Beilstein:
5929916
EC Number:
269-991-7
MDL number:
MFCD00041777
UNSPSC Code:
12161700
PubChem Substance ID:
24891117
NACRES:
NA.25

Quality Level

200

Assay

≥99% (titration)

form

crystalline

useful pH range

8.3-9.7

pKa (25 °C)

9.0

solubility

water: 0.333 g/mL, clear to slightly hazy, colorless

application(s)

diagnostic assay manufacturing

SMILES string

CC(C)(CO)NCC(O)CS(O)(=O)=O

InChI

1S/C7H17NO5S/c1-7(2,5-9)8-3-6(10)4-14(11,12)13/h6,8-10H,3-5H2,1-2H3,(H,11,12,13)

InChI key

ACERFIHBIWMFOR-UHFFFAOYSA-N

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General description

N-(1,1-dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid (AMPSO) is used as commercial pH buffers.

Application

AMPSO has been used in electroblotting the electrophoresed protein to nitrocellulose.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Quantitative analysis of GAP-43 expression by neurons in microcultures using cell-ELISA
Schreyer DJ, et al.
Journal of Neuroscience Methods, 72(2), 137-145 (1997)
Determination of the stability constants of Pb-(DIPSO) x-(OH) y and Pb-(AMPSO) x-(OH) y systems
Eliat EM, et al.
Journal of Coordination Chemistry, 66(20), 3544-3560 (2013)
S A Bursakov et al.
Biochemical and biophysical research communications, 239(3), 816-822 (1997-11-21)
Some sulfate reducing bacteria can induce nitrate reductase when grown on nitrate containing media being involved in dissimilatory reduction of nitrate, an important step of the nitrogen cycle. Previously, it was reported the purification of the first soluble nitrate reductase
D P McGregor et al.
BioTechniques, 21(3), 463-466 (1996-09-01)
The effect of buffer composition on simultaneous PCR amplification of 16S rRNA gene fragments of five bacterial species was examined using a number of different buffer systems. Tris-based PCR buffers at final concentrations of 10 mM proved unreliable. However, when
M Hosse et al.
Environmental science & technology, 35(21), 4301-4306 (2001-11-23)
Capillary electrophoresis (CE) and fluorescence correlation spectroscopy (FCS) were employed to determine electrophoretic mobilities and hydrodynamic sizes of three humic substances (IHSS aquatic fulvic acid (FA), IHSS aquatic humic acid (HA), and IHSS peat humic acid (PHA)) as a function
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