A6132
Apyrase from potatoes
ATPase ≥3.0 units/mg protein, lyophilized powder
Synonym(s):
Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
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About This Item
biological source
potato
Quality Level
type
Grade I
form
lyophilized powder
ATPase activity
≥3.0 units/mg protein
composition
Protein, 30-60%
storage temp.
−20°C
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Application
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.
Reaction: ATP → ADP+Pi → AMP+2Pi.
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Packaging
Sold on the basis of ATPase units.
Other Notes
Mixture of high and low ratio isoenzymes.
Unit Definition
One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C
Physical form
Partially purified, lyophilized powder containing potassium succinate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
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Qian Wang et al.
Neuroscience bulletin, 37(9), 1303-1313 (2021-06-06)
Food deprivation can rescue obesity and overweight-induced mood disorders, and promote mood performance in normal subjects. Animal studies and clinical research have revealed the antidepressant-like effect of calorie restriction, but little is known about the mechanism of calorie restriction-induced mood
Avanish Rai et al.
Bio-protocol, 11(1), e3874-e3874 (2021-03-19)
Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP;
I Shirakawa et al.
The Journal of experimental biology, 198(Pt 8), 1809-1815 (1995-08-01)
Kinesin is a motor protein that converts chemical energy derived from ATP hydrolysis into mechanical work to transport cellular components along microtubules. We studied the properties of ATP-dependent microtubule-kinesin sliding with two different in vitro assay systems. In one assay
Robert A Amos et al.
Nature plants, 8(11), 1289-1303 (2022-11-11)
Rhamnogalacturonan I (RG-I) is a major plant cell wall pectic polysaccharide defined by its repeating disaccharide backbone structure of [4)-α-D-GalA-(1,2)-α-L-Rha-(1,]. A family of RG-I:Rhamnosyltransferases (RRT) has previously been identified, but synthesis of the RG-I backbone has not been demonstrated in
J Vilmart-Seuwen et al.
The Journal of cell biology, 103(4), 1279-1288 (1986-10-01)
We have tried to specify a widespread hypothesis on the requirement of ATP for exocytosis (membrane fusion). With Paramecium tetraurelia cells, synchronously (approximately 1 s) exocytosing trichocysts, ATP pools have been measured in different strains, including wild type cells, "non-discharge"
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