A5968
Anti-AP-1 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Ap-1 Antibody, Ap1 Antibody, Ap1 Antibody - Anti-AP-1 antibody produced in rabbit, Anti-c-Jun
Product Name
Anti-AP-1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 39 kDa
species reactivity
human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formalin-fixed, paraffin-embedded sections of human colon carcinoma
microarray: suitable
western blot: 1:200 using HeLa nuclear extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... JUN(3725)
Application
Anti-AP-1 antibody has been used in
- immunohistochemistry
- immunochemistry
- western blotting
Immunofluorescence of Hela cells was performed using monoclonal anti-AP1 (clone 100/3) as the primary antibody.
Biochem/physiol Actions
Activation Protein transcription factor 1 (AP-1) recognizes a specific sequence (TPA response element, a palindromic TRE sequence TGA(C/G) TCA or related sequences) present in many promoter and enhancer gene regions. Regulation of AP-1 is achieved by changes in the expression of Jun and Fos proteins in the dimer, by phosphorylation at both Ser63 and Ser73 by c-Jun N-terminal kinases (JNK) and by interaction with a variety of transcriptional coactivators like C-Jun activation domain-binding protein-1 (JAB1). AP-1 is induced by a variety of signals including those eliciting proliferation, differentiation and apoptosis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Activation Protein transcription factor 1 (AP-1) is a leucine zipper transcription factor that recognizes a specific palindromic sequence present in several promoters and enhancers. AP-1 can be induced by several growth and apoptosis-related signaling pathways. Jun proteins are also involved in metastatic, inflammatory and stress responses.
Anti-AP-1/c-Jun recognizes an epitope located on the c-Jun DNA binding domain. This epitope is highly conserved in c-Jun, Jun B and Jun D proteins of chicken, mouse, rat, and human. By immunoblotting, the antibody reacts specifically with AP-1/c-Jun (a single band or occasionally a doublet at 39kDa region). Additional bands of lower molecular weight may be observed. Staining of AP-1/c-Jun band(s) is inhibited by the AP-1/c-Jun immunizing peptide (amino acid residues 246-263).
Anti-AP-1/c-Jun recognizes an epitope located on the c-Jun DNA binding domain. This epitope is highly conserved in c-Jun, Jun B and Jun D proteins of chicken, mouse, rat, and human. By immunoblotting, the antibody reacts specifically with AP-1/c-Jun (a single band or occasionally a doublet at 39kDa region). Additional bands of lower molecular weight may be observed. Staining of AP-1/c-Jun band(s) is inhibited by the AP-1/c-Jun immunizing peptide (amino acid residues 246-263).
Immunogen
synthetic peptide corresponding to amino acids 246-263 of human c-Jun with N-terminally added lysine, conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
AP-1 function and regulation
Karin M, et al.
Current Opinion in Cell Biology, 9(2), 240-246 (1997)
P Angel et al.
Nature, 332(6160), 166-171 (1988-03-10)
Proto-oncogenes encode proteins with three main sites of action: the cell-surface membrane, the cytoplasm and the nucleus. Although the exact biochemical function of most proto-oncogene products is not understood, several of them are known to be involved in signal transduction.
Vasilios P Koulouras et al.
International journal of clinical and experimental medicine, 4(1), 53-66 (2011-03-12)
Recent animal studies have demonstrated that pre-treatment with inhaled carbon monoxide (iCO) exert anti-inflammatory effects in various septic models. In all these models, there is no information whether iCO might act therapeutically after the onset of septic damage. The objective
Samuel Jang et al.
Cancer medicine, 6(9), 2142-2152 (2017-08-05)
Notch signaling is minimally active in neuroendocrine (NE) cancer cells. While histone deacetylase inhibitors (HDACi) suppress NE cancer growth by inducing Notch, the molecular mechanism underlying this interplay has not yet been defined. NE cancer cell lines BON, H727, and
Ui Joung Youn et al.
Biological & pharmaceutical bulletin, 34(6), 890-893 (2011-06-02)
Tumor necrosis factor α (TNF-α), which is a primary cytokine responsible for inflammatory responses in skin, induces the synthesis of matrix metalloproteinase-9 (MMP-9), which causes skin aging. The protective effects of 3-deoxysappanchalcone against TNF-α-induced damage was investigated using human skin
Related Content
Datasheet
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service