A5963
Anti-c-Myc−Alkaline Phosphatase antibody, Mouse monoclonal
clone 9E10, purified from hybridoma cell culture
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Synonym(s):
Anti-c-Myc, Monoclonal Anti-c-Myc−Alkaline Phosphatase antibody produced in mouse
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9E10, monoclonal
form
buffered aqueous glycerol solution
species reactivity
human
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1:100 using a myc-tagged fusion protein.
isotype
IgG1
UniProt accession no.
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... MYC(4609)
Related Categories
General description
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. The c-myc gene encodes for a protein of 62kDa called p62c-myc is associated mainly with cell nuclei.
Immunogen
synthetic peptide of the human p62c-Myc protein.
Application
Anti-c-Myc-Alkaline Phosphatase antibody has been used in
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemical labeling
- immunostaining
- western blotting
Monoclonal Anti-c-Myc-Alkaline Phosphatase antibody can be used for western blot assays at 1:100, using myc-tagged fusion proteins. The antibody can also be used for immunohistochemical applications.
Biochem/physiol Actions
The c-myc oncogene product(p62c-myc) exerts normal and oncogenic cellular functions. Elevated level of c-myc protein in malignant tissues when compared with normal tissue, but with the unexpected finding of a cytoplasmic accumulation of the protein in these tumors.
Physical form
Solution in 0.05 M Tris buffer, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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