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sterility
sterile-filtered
pH
6.0-8.0
storage temp.
2-8°C
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Application
Ampholytes for use with polyacrylamide, agarose and dextran gels, or density gradient stabilizing media in analytical and preparative isoelectric focusing.
Other Notes
IEF carrier ampholytes
Legal Information
Ampholine is a registered trademark of Cytiva
Regulatory Information
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Yu Liang et al.
Electrophoresis, 30(23), 4034-4039 (2009-12-05)
Monolithic immobilized pH gradient (M-IPG) materials were prepared in microchannles by photoinitiated polymerization of acrylamide, glycidylmethacrylate and Bis, followed by the attachment of focused Ampholine onto the surface of porous monoliths via epoxide groups. With M-IPG materials as matrix, FITC-labeled
A B Chen et al.
Journal of chromatography. A, 744(1-2), 279-284 (1996-09-13)
Four commercial ampholytes: Ampholine and Pharmalyte (Pharmacia Biotech), Bio-Lyte (Bio-Rad) and Servalyt (Serva) were evaluated for their ability to resolve recombinant tissue-type plasminogen activator (rt-PA) glycoforms by isoelectric focusing (IEF) and capillary IEF (cIEF). Each brand of ampholytes focused rt-PA
C Van der Heul et al.
Clinical science (London, England : 1979), 60(2), 185-190 (1981-02-01)
1. According to the Fletcher-Huehns hypothesis there exists a functional difference between the two iron-binding sites of transferrin. 2. The aim of the study presented was to evaluate this hypothesis in a homogeneous system, with human bone marrow cells and
Chun Yang et al.
Electrophoresis, 25(12), 1729-1734 (2004-06-24)
Glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) were used to synthesize a monolithic capillary column containing reactive epoxy groups. Glutaraldehyde was introduced and linked to the monolith after a process of amination. An aqueous solution of commercial carrier ampholytes (CAs
A Oratore et al.
The Biochemical journal, 259(3), 909-912 (1989-05-01)
We have investigated the effect in solution of synthetic carrier ampholytes on the saturation of human serum transferrin. By spectrophotometric titrations of human serum transferrin with various Fe3+-carrier ampholyte solutions, we demonstrated that under these conditions carrier ampholytes behave as
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