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A5852

Sigma-Aldrich

Alkaline Phosphatase Stop Solution

for ELISA

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UNSPSC Code:
12352204
NACRES:
NA.83

Quality Level

form

powder

solubility

water: 100 mL, clear, colorless

storage temp.

2-8°C

Application

Stop solution for Alkaline Phosphatase Red and Blue microwell substrate solutions. Use 50 μl of stop solution per 200 μl of reaction mix. Alkaline Phosphatase Stop Solution has been used in terminating the alkaline phosphatase and nitrotetrazolium blue chloride (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) reaction on nitrocellulose membrane.

Reconstitution

Reconstitute with 100 mL of water and mix for 30-60 min.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter.
Nallaseth FS and Anderson S
Microbial cell factories, 12(1), 36-36 (2013)

Articles

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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