Recommended Products
sterility
sterile-filtered
pH
5.0-7.0
storage temp.
2-8°C
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Application
Ampholytes for use with polyacrylamide, agarose and dextran gels, or density gradient stabilizing media in analytical and preparative isoelectric focusing.
Other Notes
IEF carrier ampholytes
Legal Information
Ampholine is a registered trademark of Cytiva
Regulatory Information
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Chun Yang et al.
Electrophoresis, 25(12), 1729-1734 (2004-06-24)
Glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) were used to synthesize a monolithic capillary column containing reactive epoxy groups. Glutaraldehyde was introduced and linked to the monolith after a process of amination. An aqueous solution of commercial carrier ampholytes (CAs
M Kuwada et al.
Preparative biochemistry & biotechnology, 30(2), 125-132 (2000-05-04)
The purification process for cytochrome P450 is very complicated, involving five or more column chromatography steps for the final preparation. This paper describes a reduction in the number of the steps; it can be easily purified from pig testis microsomes
Csilla Páger et al.
Electrophoresis, 32(14), 1875-1884 (2011-07-20)
Capillary isoelectric focusing in the presence of electroosmosis with sequential injection of carrier ampholytes and sample was found to be suitable for MS detection. The separate injection of the sample and the ampholytes provides good condition to suppress and overcome
A Oratore et al.
The Biochemical journal, 259(3), 909-912 (1989-05-01)
We have investigated the effect in solution of synthetic carrier ampholytes on the saturation of human serum transferrin. By spectrophotometric titrations of human serum transferrin with various Fe3+-carrier ampholyte solutions, we demonstrated that under these conditions carrier ampholytes behave as
Pier Giorgio Righetti et al.
Electrophoresis, 28(21), 3799-3810 (2007-10-09)
The present review summarizes the data accumulated in 1-year work, by exploring, via a 3-D methodology (Rotofor fractionation followed by CE-MS), all the narrow (2 pH unit wide) carrier ampholyte (CA) compounds for IEF, produced by three companies (Pharmacia with
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