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Merck
CN

A5588

Anti-polyHistidine−Alkaline Phosphatase antibody, Mouse monoclonal

clone HIS-1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-polyHistidine, Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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About This Item

NACRES:
NA.56
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-polyHistidine−Alkaline Phosphatase antibody, Mouse monoclonal, clone HIS-1, purified from hybridoma cell culture

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

buffered aqueous glycerol solution

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Application

Antibody is suitable for ELISA, immunoblotting and dot blotting assays.

Biochem/physiol Actions

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

General description

The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000430829
0000430829
0000389409
0000389409
0000388870

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Richard Glatz et al.
The Journal of biological chemistry, 278(22), 19743-19750 (2003-03-20)
Polydnaviruses are essential for the survival of many Ichneumonoid endoparasitoids, providing active immune suppression of the host in which parasitoid larvae develop. The Cotesia rubecula bracovirus is unique among polydnaviruses in that only four major genes are detected in parasitized
Min Yang et al.
Nature chemical biology, 14(12), 1109-1117 (2018-11-14)
The elucidation and prediction of how changes in a protein result in altered activities and selectivities remain a major challenge in chemistry. Two hurdles have prevented accurate family-wide models: obtaining (i) diverse datasets and (ii) suitable parameter frameworks that encapsulate
Sarah E Partovi et al.
The Journal of biological chemistry, 293(14), 5236-5246 (2018-02-08)
For nearly 30 years, coenzyme M (CoM) was assumed to be present solely in methanogenic archaea. In the late 1990s, CoM was reported to play a role in bacterial propene metabolism, but no biosynthetic pathway for CoM has yet been
Pooja Bhardwaj et al.
Antimicrobial agents and chemotherapy, 60(4), 2209-2221 (2016-01-27)
Chlorhexidine is a bisbiguanide antiseptic used for infection control. Vancomycin-resistantE. faecium(VREfm) is among the leading causes of hospital-acquired infections. VREfm may be exposed to chlorhexidine at supra- and subinhibitory concentrations as a result of chlorhexidine bathing and chlorhexidine-impregnated central venous
A short disordered N-terminal flagellin segment is a fully functional flagellar type III export signal. Application for secreted protein expression
Dobo,J et al.
Applied and Environmental Microbiology (2009)
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