A5230
Anti-ARF6 antibody, Mouse monoclonal
clone ARFAG, purified from hybridoma cell culture
Synonym(s):
ARF6 Antibody - Anti-ARF6 antibody, Mouse monoclonal, Arf6 Antibody, Anti-ADP-Ribosylation Factor 6
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ARFAG, monoclonal
form
buffered aqueous solution
mol wt
antigen 18 kDa
species reactivity
bovine, canine, rat, human
concentration
2 mg/mL
technique(s)
western blot: 1-2 μg/mL using MDCK total cell extract.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ARF6(382)
rat ... Arf6(79121)
General description
ARF6 is a member of the ADP-ribosylation factor (ARF) family. It is specifically involved in endocytosis of plasma membrane proteins.
Anti-ARF6 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ARFAG produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. class III includes only human Arf6. The ADP-ribosylation factors (Arf) family of proteins, which belong to the Ras (rat sarcoma viral oncogene homolog) family, are low molecular weight proteins capable of binding GTP.
Application
Anti-ARF6 antibody, Mouse monoclonal has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Mouse Monoclonal Anti-ARF6 antibody can be used for western blot assays at 1-2μg/ml using MDCK cell extracts. The antibody can also be used for ELISA and immunocytochemistry.
Biochem/physiol Actions
ADP-ribosylation factor 6 (Arf6) is present at the plasma membrane and on endosomal membranes where it regulates the flow of trafficking into and out of the cell, and regulates the actin cytoskeleton on the plasma membrane. ARF6 activates phospholipase D (PLD) and phosphatidylinositol 4-phosphate 5-kinase (PIP5K) to generate phosphatidic acid and PIP2 (phosphotidylinositol 4,5-bisphosphate), respectively.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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The Arf6 activator Efa6/PSD3 confers regional specificity and modulates ethanol consumption in Drosophila and humans
Gonzalez DA, et al.
Molecular Psychiatry, 23(3), 621-621 (2018)
Summer F Acevedo et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(46), 15396-15402 (2015-11-21)
Alcohol use disorders (AUDs) affect people at great individual and societal cost. Individuals at risk for AUDs are sensitive to alcohol's rewarding effects and/or resistant to its aversive and sedating effects. The molecular basis for these traits is poorly understood.
Beatriz Benayas et al.
Journal of extracellular biology, 2(7), e100-e100 (2024-06-28)
Interest in the use of extracellular vesicles (EVs) as biomarkers of disease is rapidly growing. However, one main unsolved issue in the EV field is finding a technique able to eliminate non-EV contaminants present in biofluid samples in a one-step
D A Gonzalez et al.
Molecular psychiatry, 23(3), 621-628 (2017-06-14)
Ubiquitously expressed genes have been implicated in a variety of specific behaviors, including responses to ethanol. However, the mechanisms that confer this behavioral specificity have remained elusive. Previously, we showed that the ubiquitously expressed small GTPase Arf6 is required for
Multiple Roles for Arf6: Sorting, Structuring, and Signaling at the Plasma Membrane
Donaldson JG
The Journal of biological chemistry, 278(43), 41573-41576 (2003)
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