A5174
Ampholine®
pH 3.5-10.0
pH
3.5-10.0
sterility
sterile-filtered
storage temp.
2-8°C
Application
Ampholytes for use with polyacrylamide, agarose and dextran gels, or density gradient stabilizing media in analytical and preparative isoelectric focusing.
Other Notes
IEF carrier ampholytes
Legal Information
Ampholine is a registered trademark of Cytiva
Regulatory Information
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C Van der Heul et al.
Clinical science (London, England : 1979), 60(2), 185-190 (1981-02-01)
1. According to the Fletcher-Huehns hypothesis there exists a functional difference between the two iron-binding sites of transferrin. 2. The aim of the study presented was to evaluate this hypothesis in a homogeneous system, with human bone marrow cells and
Pier Giorgio Righetti et al.
Electrophoresis, 28(21), 3799-3810 (2007-10-09)
The present review summarizes the data accumulated in 1-year work, by exploring, via a 3-D methodology (Rotofor fractionation followed by CE-MS), all the narrow (2 pH unit wide) carrier ampholyte (CA) compounds for IEF, produced by three companies (Pharmacia with
Yu Liang et al.
Electrophoresis, 30(23), 4034-4039 (2009-12-05)
Monolithic immobilized pH gradient (M-IPG) materials were prepared in microchannles by photoinitiated polymerization of acrylamide, glycidylmethacrylate and Bis, followed by the attachment of focused Ampholine onto the surface of porous monoliths via epoxide groups. With M-IPG materials as matrix, FITC-labeled
Csilla Páger et al.
Electrophoresis, 32(14), 1875-1884 (2011-07-20)
Capillary isoelectric focusing in the presence of electroosmosis with sequential injection of carrier ampholytes and sample was found to be suitable for MS detection. The separate injection of the sample and the ampholytes provides good condition to suppress and overcome
M Kuwada et al.
Preparative biochemistry & biotechnology, 30(2), 125-132 (2000-05-04)
The purification process for cytochrome P450 is very complicated, involving five or more column chromatography steps for the final preparation. This paper describes a reduction in the number of the steps; it can be easily purified from pig testis microsomes
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