A5170
Anti-Avidin antibody produced in rabbit
whole antiserum
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
contains
15 mM sodium azide
technique(s)
immunoelectrophoresis: suitable
indirect ELISA: 1:100,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties. Avidin from corn has low molecular weight than chicken egg-derived avidin. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
Immunogen
avidin
Application
Anti-Avidin antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in preparation of antibody-Au nanoparticles.
The presence of Scavidin in BT4C glioma cells was assessed by western blot using a rabbit anti-avidin antibody as the primary at a dilution of 1:4000. Lehtolainen, P. (2002) Cloning and Characterization of Scavidin, a Fusion Protein for the Targeted Delivery of Biotinylated Molecules. J. Biol. Chem. 277:8545-8550.
Biochem/physiol Actions
Avidin binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Physical form
Supplied as a liquid containing 15mM sodium azide as preservative
Preparation Note
treated to remove lipoproteins
Analysis Note
This antisera is evaluated for performance and specificity by immunodiffusion and immunoelectrophoresis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Coomassie Brilliant Dyes as Surface-Enhanced Raman Scattering Probes for Protein-Ligand Recognitions
Han, Xiao X. and others
Analytical Chemistry, 82(10), 4102-4106 (2010)
Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects
Abouseadaa HH, et al.
BMC plant biology, 15(1), 1-8 (2015)
Plant molecular farming: systems and products
Horn ME, et al.
Plant Cell Reports, 22(10), 711-720 (2004)
Heba H Abouseadaa et al.
BMC plant biology, 15, 183-183 (2015-07-22)
Wheat is considered the most important cereal crop all over the world. The wheat weevil Sitophilus granarius is a serious insect pests in much of the wheat growing area worldwide and is responsible for significant loss of yield. Avidin proteins
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(4), 577-580 (1981-04-01)
The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the
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