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A50120

Sigma-Aldrich

DEAE–Sephadex®

Synonym(s):

Diethylaminoethyl Sephadex®

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About This Item

CAS Number:
39455-31-7
MDL number:
MFCD00132778
UNSPSC Code:
47101511
NACRES:
NA.56

form

powder

Quality Level

200

technique(s)

protein purification: suitable

matrix

cross-linked dextran

bead size

40-125 μm (dry)

pore size

~200,000 Da exclusion limit

pH

2—12

capacity

3-4 meq/g binding capacity

Related Categories

General description

A50120-100G′s updated product number is GE17-0180-01
A50120-500G′s updated product number is GE17-0180-02

Application

DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. In has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000324823
0000307352
0000307352
0000324823
0000267114

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the procedure for swelling Product A50120, DEAE-Sephadex® resin?

    Weigh out the required amount of dry powder and suspend it in the buffer of choice. Although the swelling volume will be specific to the binding buffer used, generally 1 gram of powder will swell to 25 mL final volume of medium. Excess binding buffer should be used. Swell the resin for 1-2 days at room temperature or 2 hours at 100°C over boiling water. After swelling, wash the resin on a Buchner funnel with binding buffer. Prepare a slurry with 75% swelled medium/25% binding buffer.

  4. What procedures are used for elution when using Product A50120, DEAE-Sephadex® resin?

    For DEAE Sephadex, elution is achieved using either an increasing salt gradient or a decreasing pH gradient.

  5. How can Product A50120, DEAE-Sephadex® resin be regenerated?

    Regeneration is generally accomplished by washing with high ionic strength buffer or decreasing/increasing pH. The resin is then re-equilibrated in binding buffer.

  6. How can Product A50120, DEAE-Sephadex® resin, be stored?

    Store swollen medium refrigerated in the presence of a preservative such as 20% Ethanol. Sodium azide or thimerosal should not be used.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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E Okuno et al.
Biochimica et biophysica acta, 841(1), 112-119 (1985-07-26)
Quinolinic acid phosphoribosyltransferase (EC 2.4.2.19) was purified 3600-fold from rat liver and 280-fold from rat brain. Kinetic analyses (Km = 12 microM for the substrate quinolinic acid and Km = 23 microM for the cosubstrate phosphoribosylpyrophosphate), physicochemical properties of the
Misook Kim et al.
Biochimica et biophysica acta, 1770(12), 1627-1635 (2007-10-09)
Two cysteine proteases, GP2 and GP3, have been isolated from ginger rhizomes (Zingiber officinale). GP2 is virtually identical to a previously identified ginger protease GPII [K.H. Choi, and R.A. Laursen, Amino-acid sequence and glycan structures of cysteine proteases with proline
P K Farmer et al.
Biochimica et biophysica acta, 1434(1), 6-17 (1999-11-11)
A calmodulin-like domain protein kinase (DcCPK1, previously designated CDPK431) cloned from carrot (Daucus carota L.) was expressed at high levels in Escherichia coli and partially purified. Ca(2+)-induced gel mobility shift and (45)Ca(2+) ligand binding assays confirmed that recombinant DcCPK1 binds
M A Zaval'nyĭ et al.
Voprosy virusologii, (5)(5), 583-589 (1980-09-01)
Primary cells of chick embryos (CEC), new-born piglet kidneys (NPK), and green monkey kidney (GMK) obtained by the conventional method of tissue trypsinization were shown to grow satisfactorily on microcarriers of various types. CEC formed a confluent monolayer on Supperbead
Clovis R Nakaie et al.
Analytical biochemistry, 318(1), 39-46 (2003-06-05)
This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports can be used for peptide synthesis. Moreover, due to the high stability of
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