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A50120

Sigma-Aldrich

DEAE–Sephadex®

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Synonym(s):
Diethylaminoethyl Sephadex®
CAS Number:
39455-31-7
MDL number:
MFCD00132778
UNSPSC Code:
47101511
NACRES:
NA.56

form

powder

Quality Level

200

technique(s)

protein purification: suitable

matrix

cross-linked dextran

bead size

40-125 μm (dry)

pore size

~200,000 Da exclusion limit

pH

2—12

capacity

3-4 meq/g binding capacity

Related Categories

General description

A50120-100G′s updated product number is GE17-0180-01
A50120-500G′s updated product number is GE17-0180-02

Application

DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. In has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the procedure for swelling Product A50120, DEAE-Sephadex® resin?

    Weigh out the required amount of dry powder and suspend it in the buffer of choice. Although the swelling volume will be specific to the binding buffer used, generally 1 gram of powder will swell to 25 mL final volume of medium. Excess binding buffer should be used. Swell the resin for 1-2 days at room temperature or 2 hours at 100°C over boiling water. After swelling, wash the resin on a Buchner funnel with binding buffer. Prepare a slurry with 75% swelled medium/25% binding buffer.

  4. What procedures are used for elution when using Product A50120, DEAE-Sephadex® resin?

    For DEAE Sephadex, elution is achieved using either an increasing salt gradient or a decreasing pH gradient.

  5. How can Product A50120, DEAE-Sephadex® resin be regenerated?

    Regeneration is generally accomplished by washing with high ionic strength buffer or decreasing/increasing pH. The resin is then re-equilibrated in binding buffer.

  6. How can Product A50120, DEAE-Sephadex® resin, be stored?

    Store swollen medium refrigerated in the presence of a preservative such as 20% Ethanol. Sodium azide or thimerosal should not be used.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

J P Rapp et al.
Hypertension (Dallas, Tex. : 1979), 6(4), 519-525 (1984-07-01)
Dahl salt-sensitive (S) rats which are susceptible to hypertension have lower urinary kallikrein excretion than salt-resistant (R) rats which are not susceptible. Some physicochemical characteristics of partially purified urinary kallikrein were compared between the S and R strains. The isoelectric
C Raghothama et al.
Clinica chimica acta; international journal of clinical chemistry, 264(1), 13-25 (1997-08-08)
Glycated hemoglobin can be degraded by proteolytic enzyme(s) in the erythrocyte. The enzyme(s) co-elutes with glycated hemoglobin when the latter is separated from erythrocyte lysates using the cation-exchanger Bio Rex-70. A further purification of the Bio Rex eluant on DEAE
R Hao et al.
The Journal of biological chemistry, 266(8), 5135-5139 (1991-03-15)
L-Glutamate decarboxylase, an enzyme under the control of the asexual developmental cycle of Neurospora crassa, was purified to homogeneity from conidia. The purification procedure included ammonium sulfate fractionation and DEAE-Sephadex and cellulose phosphate column chromatography. The final preparation gave a
D A Schafer et al.
Biochemical and biophysical research communications, 115(3), 807-813 (1983-09-30)
A single form of cytochrome b5 has been isolated in highly purified form from the cytosolic fraction of rabbit erythrocytes by sequential chromatography on DE-52 cellulose, Sephadex G-75, and DEAE-Sephadex A50. The cytochrome is structurally similar to the N-terminal, heme-binding
Effect of heme orientation on the reduction potential of cytochrome b5.
F A Walker et al.
Journal of the American Chemical Society, 110(18), 6234-6240 (1988-08-01)
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