A4843
Anti-FITC−Alkaline Phosphatase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous glycerol solution
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About This Item
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (i), IHC (p)
Citations:
4
biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
technique(s)
dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues, indirect ELISA: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
FITC (fluorescein isothiocyanate) is a fluorescent dye that is often used to label antibodies in immunofluorescent assays. As FITC does not interfere with the biological action of proteins, it can be used as a hapten to label different proteins. FITC conjugation mainly facilitates the accurate detection and localization of proteins in immunological techniques
Anti-FITC-Alkaline Phosphatase antibody is specific for free FITC and FITC conjugated to various biomolecules such as proteins, oligonucleotides, and nucleic acids.
Anti-FITC-Alkaline Phosphatase antibody is specific for free FITC and FITC conjugated to various biomolecules such as proteins, oligonucleotides, and nucleic acids.
Immunogen
FITC-BSA
Application
2% paraformaldhyde fixed paraffin embedded human rectal sections were stained with alkaline phosphatase-conjugated rabbit anti-FITC at 1:100.
Anti-FITC-Alkaline Phosphatase antibody is suitable for use in fluorescence in situ hybridization at 1:100 or 1:200 dilutions.
Biochem/physiol Actions
The purified IgG fraction of antiserum is adsorbed to remove any antibodies to BSA and then conjugated to alkaline phosphatase. The conjugate may be used for detection of FITC labeled moieties and for visualization of immunofluorescence by light microscopy.
Physical form
Solution in 0.05 M Tris buffer, containing 1% BSA, 50% glycerol and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
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Oscar R Lara et al.
Methods in molecular biology (Clifton, N.J.), 295, 301-310 (2004-12-15)
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P Heukels et al.
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