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A4605

Sigma-Aldrich

Anti-iASPP antibody, Mouse monoclonal

clone LXO49.3, purified from hybridoma cell culture

Synonym(s):

Anti-Inhibitory Member of the Apoptosis-stimulating Protein of p53 Family, Anti-RAI, Anti-Rel-associated Inhibitor

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LXO49.3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~100 kDa

species reactivity

mouse, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of human osteogenic sarcoma (U-2-OS)

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Monoclonal Anti-iASPP (mouse IgG1 isotype) is derived from the hybridoma LXO49, produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding residues of human iASPP. The ASPP family of proteins contains three members, ASSP1, ASSP2 and iASPP. iASPP is also known as REL associated inhibitor (RAI). iASPP exists as a 100 kDa form and as a 70 kDa form (iASPP/RAI). It is found both in the nucleus and cytoplasm, while iASPP/RAI is mainly cytosolic. PPP1R13L (protein phosphatase 1 regulatory subunit 13 like) codes for iASPP, that is located on human chromosome 19q13.
iASPP is an inhibitory member of the apoptosis-stimulating protein of p53 (ASPP) family. It is an oncoprotein that associates with Ras, E1A and E7 to transform cells.

Application

Monoclonal Anti-iASPP antibody produced in mouse has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation

Mouse monoclonal Anti-iASPP antibody can be used for western blot (0.1-0.2μg/mL using total cell extract of human osteogenic sarcoma), microarray, immunoprecipitation and immunocytochemistry.

Biochem/physiol Actions

PPP1R13L (protein phosphatase 1 regulatory subunit 13 like)/iASPP inhibits p53-mediated cell death. It interacts and inhibits NFkB p65 RelA. It may act as a therapeutic target for bladder cancer (BC). It modulates cell proliferation and apoptosis. iASPP plays a key role in breast cancer and acute leukemia.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ariel M Wilson et al.
PloS one, 9(4), e94175-e94175 (2014-04-10)
The transcription factor p53 mediates the apoptosis of post-mitotic neurons exposed to a wide range of stress stimuli. The apoptotic activity of p53 is tightly regulated by the apoptosis-stimulating proteins of p53 (ASPP) family members: ASPP1, ASPP2 and iASPP. We
Anissa Chikh et al.
The EMBO journal, 30(20), 4261-4273 (2011-09-08)
iASPP, an inhibitory member of the ASPP (apoptosis stimulating protein of p53) family, is an evolutionarily conserved inhibitor of p53 which is frequently upregulated in human cancers. However, little is known about the role of iASPP under physiological conditions. Here
Inhibitory member of the apoptosis-stimulating protein of p53 is overexpressed in bladder cancer and correlated to its progression
Wu Z, et al.
Medicine, 96(19) (2017)
A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity
Kramer D, et al.
Cell Death & Disease, 6(2), e1634-e1634 (2015)
The miR-124-p63 feedback loop modulates colorectal cancer growth
Liu K, et al.
Oncotarget, 8(17), 29101-29101 (2017)

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