A4480
Anti-ASPP2 antibody, Mouse monoclonal
~1.5 mg/mL, clone DX 54.10, purified from hybridoma cell culture
Synonym(s):
Anti-53BP2 apoptosis-stimulating protein of p53 2, Anti-TP53BP2, Anti-tumor protein p53-binding protein 2
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DX 54.10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~175 kDa
species reactivity
human, mouse
concentration
~1.5 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of human osteogenic sarcoma
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TP53BP2(7159)
mouse ... Trp53bp2(209456)
Related Categories
General description
Monoclonal Anti-ASPP2 (mouse IgG1 isotype) is derived from the hybridoma DX 54.10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice. Apoptosis-stimulating protein of p53 (ASPP) family of proteins contains three members, ASPP1, ASPP2 and iASPP, which interact via their C-terminal region with p53 and modulate its activity.
Specificity
Monoclonal Anti-ASPP2 recognizes human and mouse ASPP2. The epitope recognized by the antibody resides within amino acids 691-1128 of human ASPP2.
Immunogen
fragment of human ASPP2 (amino acids 691-1128).
Application
Mouse Monoclonal Anti-ASPP2 has been used for immunofluorescence analysis. The antibody can also be used for immunocytochemistry, immunoprecipitation and microarray applications.
Western blot analysis of Hek293 or 3T3-L1 adipocyte cell lysates was performed using monoclonal mouse anti-ASPP2 as the primary.
Biochem/physiol Actions
ASPP2 is a tumor suppressor that activates p53. ASPP2 also regulates Par-3 which subsequently modulates the proliferation and polarity of neural progenitor cells during CNS development. Furthermore, ASPP2 interacts with active Ras to induce p53 mediated cell death. ASPP2 is known to function as a biomarker for invasive pituitary adenomas.
Apoptosis-stimulating protein of p53 (ASPP2) enhances the ability of p53 to induce apoptosis ASPP1 and ASPP2 stimulates the transactivation function of p63 and p73 on promoters of different genes such as BCL2 associated X, apoptosis regulator (Bax), p53-inducible gene 3 (PIG3) and p53 upregulated modulator of apoptosis (PUMA), but not mouse double minute 2 homolog (Mdm2) or p21WAF-1/CIP1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Lin Ma et al.
Oncology letters, 6(5), 1313-1318 (2013-11-02)
Nucleostemin is a GTP-conjugated protein located in the nucleoli of stem cells and certain cancer cells, and maintains cellular self-renewal. The present study aimed to evaluate nucleostemin as a potential target for pituitary adenoma gene therapy by investigating nucleostemin and
Roberta Sottocornola et al.
Developmental cell, 19(1), 126-137 (2010-07-14)
Cell polarity plays a key role in the development of the central nervous system (CNS). Interestingly, disruption of cell polarity is seen in many cancers. ASPP2 is a haplo-insufficient tumor suppressor and an activator of the p53 family. In this
Wei-Tzu Sun et al.
Biochemical and biophysical research communications, 376(2), 395-398 (2008-09-17)
The p53 tumor suppressor functions in maintaining the integrity of the genome. We have previously reported that DDA3 is an oncoprotein transcriptionally regulated by p53. To explore mechanisms underlying DDA3 action, we searched for its interacting proteins by yeast two-hybrid
ASPP proteins specifically stimulate the apoptotic function of p53
Samuels-Lev Y, et al.
Molecular Cell, 8(4), 781-794 (2001)
ASPP1 and ASPP2 bind active RAS, potentiate RAS signalling and enhance p53 activity in cancer cells.
Y Wang et al.
Cell death and differentiation, 20(4), 525-534 (2013-02-09)
RAS mutations occur frequently in human cancer and activated RAS signalling contributes to tumour development and progression. Apart from its oncogenic effects on cell growth, active RAS has tumour-suppressive functions via its ability to induce cellular senescence and apoptosis. RAS
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