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A3861

Sigma-Aldrich

Anti-Palladin (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PALLD

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~140 kDa (another band at 90 kDa)

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): 10-20 μL using HeLa cell lysates
indirect immunofluorescence: 1:250-1:500 using human HeLa cells
western blot: 1:250-1:500 using whole extracts of human HeLa cells

UniProt accession no.

Q8WX93

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PALLD(23022)
mouse ... Palld(72333)

General description

Anti-Palladin (C-terminal) is produced in rabbit using as the immunogen a synthetic peptide corresponding to a sequence at the C-terminal of human palladin, conjugated to KLH. Palladin, an actin associated Ig-domain protein is mapped to human chromosome 4q32. This phosphoprotein is widely expressed in vertebrate cells and tissues. Palladin is expressed as three major isoforms of 90, 140 and 200 kDa.

Application

Anti-Palladin (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Rabbit anti-Palladin (C-terminal) antibody can be used for immunoprecipitation (10-20μL), indirect immunofluorescence (1:250-1:500) and western blot (1:250 -1:500) using HeLa cells.

Biochem/physiol Actions

Palladin is a component of actin-containing microfilaments that control cell shape, adhesion, and contraction. Its expression is required for normal mammalian embryogenesis since inactivation of paladin leads to embryonic lethality. Palladin interacts with the actin-associated proteins -actinin, VASP, ezrin, and profilin, suggesting that it is required for normal actin cytoskeleton organization. It serves as a transcriptional regulator, which controls the expression of smooth muscle cell genes.
Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer. Rabbit anti-Palladin (C-terminal) antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide. A nonspecific band of approx. 55kDa may be detected in some cell extract preparations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
089K4752

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Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment
McLane JS and Ligon LA
Biophysical Journal, 109(2), 249-264 (2015)
Meta-analysis of sex differences in gene expression in schizophrenia
BMC Systems Biology, 10(1), S9-S9 (2016)
The actin associated protein palladin is important for the early smooth muscle cell differentiation
Jin L, et al.
PLoS ONE, 5(9), e12823-e12823 (2010)
Kay L Pogue-Geile et al.
PLoS medicine, 3(12), e516-e516 (2006-12-30)
Pancreatic cancer is a deadly disease. Discovery of the mutated genes that cause the inherited form(s) of the disease may shed light on the mechanism(s) of oncogenesis. Previously we isolated a susceptibility locus for familial pancreatic cancer to chromosome location
Characterization of human palladin, a microfilament-associated protein
Mykka?nen OM, et al.
Molecular Biology of the Cell, 12(10), 3060-3073 (2001)
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