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A3699

Sigma-Aldrich

Acrylamide/Bis-acrylamide, 30% solution

BioReagent, suitable for electrophoresis, 37.5:1

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Synonym(s):
30% Acrylamide/Bisacrylamide 37.5:1, Polyacrylamide solution
MDL number:
MFCD00080848
UNSPSC Code:
41105319
PubChem Substance ID:
329770843
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

100

product line

BioReagent

form

liquid

feed ratio

37.5:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

storage temp.

2-8°C
2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used:
  • in the preparation of polyacrylamide gel for photobleaching experiment
  • as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
  • in the preparation of polyacrylamide gels during western blot analyses

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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