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A3606

Sigma-Aldrich

Anti-ATG3 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-APG3, Anti-APG3L, Anti-Autophagy related 3 homolog (S. cerevisiae), Anti-DKFZp564M1178, Anti-FLJ22125, Anti-MGC15201, Anti-PC3-96

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About This Item

MDL number:
MFCD09753845
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~36 kDa

species reactivity

rat, human, mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtrAvidin®-peroxidase staining of heat-retrieved sections of human cerebellum
immunoprecipitation (IP): 2-4 μL using rat NRK and mouse 3T3 cell lysates

UniProt accession no.

Q9NT62

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG3(64422)
mouse ... Atg3(67841)

General description

Rabbit Anti-ATG3 (C-terminal) antibody recognizes human, rat, and mouse Atg3 by immunoprecipitation (approx. 36kDa) and immunohistochemistry. Detection of the Atg3 staining by immunohistochemistry is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 298-314 of human ATG3, conjugated to KLH via an N-terminal cysteine residue.

Application

Rabbit Anti-ATG3 (C-terminal) antibody can be used for immunohistochemistry (1:50 -1:100) and immunoprecipitation (2-4μl) assays.

Target description

ATG3 is a member of the autophagy-related protein family and is involved in the formation of the autophagosome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Qing-Hua Cao et al.
American journal of translational research, 8(9), 3831-3847 (2016-10-12)
Autophagy-related (ATG) genes contributed to tumorigenesis and cancer progression. This study aims to investigate the expression of ATG proteins and their clinicopathological significance in gastric cancer. Nine well-known ATG proteins, (ULK1, Beclin 1, ATG3, ATG5, ATG7, ATG9, ATG10, ATG12 and
Zhiyuan You et al.
Autophagy, 15(8), 1309-1321 (2019-02-16)
TP53INP2/DOR (tumor protein p53-inducible nuclear protein 2) contributes to mammalian macroautophagy/autophagy by carrying nuclear deacetylated MAP1LC3/LC3 to the cytoplasm. Here, we report that in the cytoplasm, TP53INP2 further functions in autophagosome biogenesis by promoting LC3B-ATG7 interaction. Cytoplasmic expression of the

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