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A3422

Sigma-Aldrich

Achromopeptidase from bacteria

partially purified powder, ≥20,000 units/mg solid

Synonym(s):

ACP, Achromopeptidase

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About This Item

Enzyme Commission number:
3.4.21.50 (BRENDA, IUBMB)
MDL number:
MFCD00130333
UNSPSC Code:
12352204
NACRES:
NA.54

form

partially purified powder

Quality Level

200

specific activity

≥20,000 units/mg solid

mol wt

27 kDa

foreign activity

Collagenase, present

storage temp.

−20°C

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Application

Achromopeptidase from bacteria may be used:
  • in the homogenization step for genomic DNA extraction from microbial biofilm
  • in the lysis of gram-positive strains(
  • for DNA extraction from Nocardia strains

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Biochem/physiol Actions

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.

Unit Definition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Physical form

Partially purified powder.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P342 + P311

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Microbial ecosystem analysis in root canal infections refractory to endodontic treatment
Henriques LC, et al.
Journal of Endodontics, 42(8), 1239-1245 (2016)
Metagenomic analysis of an ecological wastewater treatment plant?s microbial communities and their potential to metabolize pharmaceuticals
Balcom IN, et al.
F1000Research, 5, 844-846 (2016)
Near real-time inhibitive assay for heavy metals using achromopeptidase
Shukor MY, et al.
Indian Journal of Biotechnology, 13, 398-403 (2014)
Achromopeptidase for lysis of anaerobic gram-positive cocci.
Ezaki T and Suzuki S
Journal of Clinical Microbiology, 16(5), 844-846 (1982)
High Intraspecific Genetic Diversity of Nocardia brasiliensis, a Pathogen Responsible for Cutaneous Nocardiosis Found in France: Phylogenetic Relationships by Using sod and hsp65 Genes
Kosova-Maali D, et al.
BioMed Research International, 2018 (2018)
View All Related Papers

Protocols

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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