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A3219

Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)

Liquid Substrate System

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Synonym(s):
ABTS
MDL number:
MFCD00214141
UNSPSC Code:
12352204
PubChem Substance ID:
24890730
NACRES:
NA.83

Quality Level

200

form

liquid

storage temp.

2-8°C

SMILES string

CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O

InChI

1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-

InChI key

ZTOJFFHGPLIVKC-CLFAGFIQSA-N

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General description

The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.

Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product. It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.

Biochem/physiol Actions

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically. A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples. ABTS method is flexible at varying pH and most widely used. ABTS is a substrate for phenol oxidase enzyme.

Physical form

Ready-to-use.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Should Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), be diluted prior to use?

    No, this product is offered as a ready-to-use (1×) solution.

  4. When using Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at what wavelength should the product of the reaction be measured?

    The product should be measured at 405 nm. For end point assays, the reaction may be stopped with 1% (w/v) SDS and also read at 405 nm.

  5. What is the concentration of Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)?

    The concentration of the ABTS is approximately 1.8 mM.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

ABTS assay of phenol oxidase activity in soil
Floch C, et al.
Journal of Microbiological Methods, 71(3), 319-324 (2007)
Wei Xin et al.
Infection and immunity, 76(7), 3241-3254 (2008-05-07)
Recombinant attenuated Salmonella vaccines (RASVs) have been used extensively to express and deliver heterologous antigens to host mucosal tissues. Immune responses can be enhanced greatly when the antigen is secreted to the periplasm or extracellular compartment. The most common method
Randall L Davis et al.
Journal of neuroimmunology, 186(1-2), 141-149 (2007-05-04)
Emerging evidence indicates that neuroinflammatory responses in astroglia, including chemokine expression, are altered by opioids. Astroglial chemokines, such as CXCL10, are instrumental in response to many neuropathological insults. Opioid mediated disruption of astroglial CXCL10 expression may be detrimental in opioid
Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis
Shalaby EA and Shanab SMM
Indian Journal of Geo-Marine Sciences, 42(5), 556-564 (2013)
A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation
Erel O
Clinical Biochemistry, 37(4), 277-285 (2004)
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