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Key Documents

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Safety Information

A2981

Sigma-Aldrich

Anti-ATG4B antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-APG4B, Anti-AUT-like 1 cysteine endopeptidase, Anti-AUTL1, Anti-Autophagin-1, Anti-Autophagy-related cysteine endopeptidase 1, Anti-Autophagy-related protein 4 homolog B, Anti-Cysteine protease ATG4B, Anti-KIAA0943, Anti-hAPG4B, ATG4

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About This Item

MDL number:
MFCD09753846
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~44 kDa

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin®-Peroxidase staining of heat-retrieved human cerebellum sections
western blot: 1:125-1:500 using whole extract of 3T3 cells
western blot: 1:500-1:1,000 using whole extract of rat brain

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product line

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application(s)

cleaning products
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food and beverages
personal care

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

compatibility

for use with (Complex cell extracts, pure protein solution, 1D and 2D PAGE gels)

compatibility

-

compatibility

-

compatibility

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

ATG4B (autophagy-related 4B cysteine peptidase), a mammalian orthologue of yeast Atg4, is a cysteine protease. It is widely expressed in human and rat tissues, being most abundant in the brain. This gene is mapped to human chromosome 2q37.
ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy.

Immunogen

synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse.

Application

Anti-ATG4B antibody produced in rabbit has been used in
  • western blotting
  • immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

ATG4B (autophagy-related 4B cysteine peptidase) participates in the modulation of autophagy in mammalian cells. It is also involved in light chain 3 (LC3) lipidation. ATG4B is responsible for the processing of the mammalian Atg8 homologues: Golgi-associated ATPase enhancer of 16 kDa (GATE16), gamma-aminobutyric-acid-type-A-receptor-associated protein (GABARAP), and LC3. It removes the carboxyl-terminal region of proLC3 immediately after its synthesis, generating the soluble LC3-I and exposing a carboxyl-terminal Gly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    21170704
    21170704
    089M4802V
    089M4802V
    097M4773V

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Let-7i-Induced Atg4B Suppression Is Essential for Autophagy of Placental Trophoblast in Preeclampsia
    Xu Y, et al.
    Journal of Cellular Physiology, 232(9), 2581-2589 (2017)
    Essential role for the ATG4B protease and autophagy in bleomycin-induced pulmonary fibrosis
    Cabrera S, et al.
    Autophagy, 11(4), 670-684 (2015)
    O-GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity
    Jo YK, et al.
    Implant Dentistry, 7(35), 57186-57186 (2016)
    The 2q37-deletion syndrome: an update of the clinical spectrum including overweight, brachydactyly and behavioural features in 14 new patients
    Leroy C, et al.
    European Journal of Human Genetics, 21(6), 602-602 (2013)
    AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells
    Ni Z, et al.
    Autophagy, 14(4), 685-701 (2018)
    View All Related Papers

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