A2859
Monoclonal Anti-ATG5 antibody produced in mouse
clone ATG5-18, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog, Anti-hAPG5
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ATG5-18, monoclonal
form
buffered aqueous solution
mol wt
antigen ~56 kDa
species reactivity
human, mouse, rat
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole extract of human K562 cells
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)
General description
Monoclonal Anti-ATG5 (mouse IgG2a isotype) is derived from the hybridoma ATG5-18 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human Atg5, conjugated to keyhole limpet hemocyanin (KLH). The autophagy related 5 (ATG5) gene is mapped to human chromosome 6q21 and encodes a 32 kDa protein.
Specificity
Mouse monoclonal anti-ATG5 antibody reacts with human, rat, and mouse Atg5-Atg12 complex by immunoblotting (∼56kDa) and immunoprecipitation.
Application
Monoclonal Anti-ATG5 antibody produced in mouse has been used in western blotting and immunoprecipitation
Omegasomes were shown to localize to autophagosomes by immunohistochemistry using rabbit anti-ATG5 antibody.
Biochem/physiol Actions
Atg5 (also known as Apg5) is a 32kDa protein that is required for mediating autophagy. Studies have reported that the Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an important role in autophagosome formation. Furthermore, a fragment of Atg5 fragment produced by calpain cleavage is known to have pro-apoptotic properties.
Autophagy related 5 (ATG5), also known as Apg5, is essential for autophagy, a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, the autophagosomes, and subsequently delivered to the lysosome for degradation and recycling. Autophagy is required for normal turnover of cellular components during starvation. It plays an essential role in cellular differentiation, cell death and aging. Defective autophagy may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Pricing
WGK
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Mireia Niso-Santano et al.
The EMBO journal, 34(8), 1025-1041 (2015-01-15)
To obtain mechanistic insights into the cross talk between lipolysis and autophagy, two key metabolic responses to starvation, we screened the autophagy-inducing potential of a panel of fatty acids in human cancer cells. Both saturated and unsaturated fatty acids such
Xian Jiang et al.
Molecular cell, 63(2), 229-239 (2016-07-23)
In response to apoptotic stimuli, mitochondria in mammalian cells release cytochrome c and other apoptogenic proteins, leading to the subsequent activation of caspases and apoptotic cell death. This process is promoted by the pro-apoptotic members of the Bcl-2 family of
Allan Sauvat et al.
EBioMedicine, 30, 261-272 (2018-04-03)
Depending on the length of their carbon backbone and their saturation status, natural fatty acids have rather distinct biological effects. Thus, longevity of model organisms is increased by extra supply of the most abundant natural cis-unsaturated fatty acid, oleic acid
Autophagy in health and disease: a double-edged sword
Shintani T and Klionsky DJ
Science (New York, N.Y.), 306(5698), 990-995 (2004)
Metastatic risk and resistance to BRAF inhibitors in melanoma defined by selective allelic loss of ATG5
Garcia F M, et al.
Autophagy, 12(10), 1776-1790 (2016)
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