A2856
Anti-ATG7 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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Synonym(s):
ATG7 Antibody - Anti-ATG7 antibody produced in rabbit, Atg7 Antibody, Anti-APG7, Anti-APG7-like, Anti-APG7L, Anti-Apoptosis-specific-protein, Anti-Autophagy protein 7-like, Anti-Ubiquitin activating enzyme E1-like protein
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~75 kDa
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μL
technique(s)
immunoprecipitation (IP): 1-2 μL using human U87 cell lysate
western blot: 0.5-1 μg/mL using whole extracts of mouse 3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ATG7(10533)
mouse ... Atg7(74244)
rat ... Atg7(312647)
Related Categories
General description
Autophagy is a catabolic process by which cell delivers cytoplasmic constituent into lysosome for protein degradation. 16 ATG genes are essential for autophagosome preparation out of which Atg7 is the one required to activate ubiquitin-like proteins Atg12 and Atg8. Atg7 is also crucial for amino acid supply in neonates, as well as for bulk degradation of proteins and organelles in mice during starvation. Anti-ATG7 antibody can be used in immunohistochemistry and immunoprecipitation. Rabbit anti-ATG7 antibody reacts specifically with Atg7 of human, mouse and rat.
Immunogen
synthetic peptide corresponding to amino acids 38-50 of human ATG7, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse and rat.
Application
Anti-ATG7 antibody can be used in western blotting and immunoblotting.
Anti-ATG7 antibody produced in rabbit has been used:
- for quantitative real-time polymerase chain reaction of autophagy-related genes
- in immunofluorescence and immunoblotting
- in in vivo acetylation/deacetylation of autophagy proteins
Rabbit anti-ATG7 antibody has been used for immunoblotting assays. The antibody is also suitable for use in immunoprecipitation applications at 1-2μL using human U87 cell lysates.
Rabbit anti-ATG7 antibody was used for immunohistochemistry on sections of mouse midbrain fixed in 4% paraformaldehyde at 1:2000.
Biochem/physiol Actions
Autophagy related 7 (ATG7) regulates caspase mediated autophagy and apoptosis. This has a significant effect on tumor progression and ATG7 acts as a tumor suppressor. ATG7 is known to be associated with many cancer types, hence it is considered as a target for cancer therapy. Loss of ATG7 gene contributes to liver carcinogenesis. The ATG7 gene encodes E1-like enzyme essential for lipidation and conjugation systems : ubiquitin-like microtubule-associated protein 1A/1B-light chain 3 (LC3) and autophagy related 12 (ATG12) respectively.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
miR-106a suppresses tumor cells death in colorectal cancer through targeting ATG7
Hao H, et al.
Medical Molecular Morphology, 50(2), 76-85 (2017)
Autophagy protects against colitis by the maintenance of normal gut microflora and mucus secretion
Tsuboi K, et al.
The Journal of Biological Chemistry (2015)
Christian Pohl et al.
Nature cell biology, 11(1), 65-70 (2008-12-17)
At the end of cytokinesis, the dividing cells are connected by an intercellular bridge, containing the midbody along with a single, densely ubiquitylated, circular structure called the midbody ring (MR). Recent studies revealed that the MR serves as a target
Altea Rocchi et al.
PLoS genetics, 13(8), e1006962-e1006962 (2017-08-15)
Impairment of the autophagy pathway has been observed during the pathogenesis of Alzheimer's disease (AD), a neurodegenerative disorder characterized by abnormal deposition of extracellular and intracellular amyloid β (Aβ) peptides. Yet the role of autophagy in Aβ production and AD
ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells
Karvela M, et al.
Autophagy, 12(6), 936-948 (2016)
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