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A2543

Sigma-Aldrich

Anti-α-Actinin antibody produced in rabbit

whole antiserum

Synonym(s):

Anti-CMD1AA, Anti-CMH23, Anti-MPD6, Anti-MYOCOZ

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1 KIT
CN¥5,282.92

CN¥5,282.92

Estimated to ship onMarch 17, 2025Details

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1 KIT
CN¥5,282.92

About This Item

MDL number:
MFCD00162107
UNSPSC Code:
12352203
NACRES:
NA.41

CN¥5,282.92

Estimated to ship onMarch 17, 2025Details

New, lower price on this item!
Request a Bulk Order

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken

technique(s)

indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: suitable using cultured cells and chicken gizzard extract

UniProt accession no.

O43707
P35609

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Gene Information

mouse ... Il1a(16175)

Gene Information

mouse ... Il9(16198)

Gene Information

mouse ... Il17b(56069)

Gene Information

mouse ... Il13(16163)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 12.23-3000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.0823-60 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 16.46-12000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8023-2000 pg/mL

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. Rabbit anti-α-actinin antibody stains α-actinin in cell cultures using indirect immunofluorescence. The antibody reacts best with cultured chicken fibroblasts, however, adequate labeling may also be obtained with cells of other species. In an immunoblot assay, the antiserum specifically stains the α-actinin band.
α-Actinin is a member of the spectrin superfamily. It consists of an antiparallel homodimer, with an N-terminal actin-binding domain (ABD), a central domain of four spectrin-like repeats (SRs), and a C-terminal calmodulin-like domain (CAMD) with two pairs of EF-hand motifs (EFs). α-Actinin exists in four isoforms: α-actinin 1, α-actinin 2, α-actinin 3 and α-actinin 4. In smooth muscles, α-actinin is present in dense bodies and plaques whereas, in normal skeletal muscles, it is associated with Z-discs that define muscle sarcomere.

Specificity

Anti-α-Actinin specifically stains α-actinin in cell cultures using indirect immunfluorescent labeling techniques. The antibody reacts best with cultured chicken fibroblasts, however adequate labeling may also be obtained with cells of other species.

Immunogen

α-actinin from chicken gizzard

Application

Anti-Actinin antibody was used to detect actinin in human mesangial cells. Cells were first grown on glass cover slips, fixed in cold methanol and permeabilized with 3.5% paraformaldehyde/0.1% Tween-20 for 5 min at room temperature.
Rabbit anti-α-actinin antibody has been used for:
  • co-immunoprecipitation studies
  • immunoblotting studies
  • immunofluorescence[1]

Biochem/physiol Actions

α-Actinin is an actin-crosslinking Z-disk protein. It plays a vital role in linking the cytoskeleton to different transmembrane proteins in a variety of junctions. α-Actinin modulates receptor activities and serves as a cytoskeleton scaffold for diverse signaling pathways. It is implicated in muscle contraction.

Physical form

Supplied as a liquid with 0.1% sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    B Sjöblom et al.
    Cellular and molecular life sciences : CMLS, 65(17), 2688-2701 (2008-05-20)
    Alpha-actinin is a cytoskeletal actin-binding protein and a member of the spectrin superfamily, which comprises spectrin, dystrophin and their homologues and isoforms. It forms an anti-parallel rod-shaped dimer with one actin-binding domain at each end of the rod and bundles
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