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A2263

α-Amanitin

Name: &#945;-Amanitin
Brand: SIGMA

From Amanita phalloides, ≥85% (HPLC), RNA polymerase II and III inhibitor, powder

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About This Item

Empirical Formula (Hill Notation):

C₃₉H₅₄N₁₀O₁₄S

CAS Number:

23109-05-9

Molecular Weight:

918.97

NACRES:

NA.32

PubChem Substance ID:

24890639

UNSPSC Code:

12352202

EC Number:

245-432-2

MDL number:

MFCD00215842

Beilstein/REAXYS Number:

1071138

Assay:

≥85% (HPLC)

Form:

powder

Quality level:

200

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Properties

Product Name

α-Amanitin, from Amanita phalloides, ≥85% (HPLC), powder

biological source

Amanita phalloides

Quality Level

200

assay

≥85% (HPLC)

form

powder

mol wt

918.97 g/mol

color

white to light yellow

mp

254-255 °C (lit.)

solubility

H₂O: 1.0 mg/mL

ε (extinction coefficient)

13,500 at 310 nm in H₂O at 1 M

storage temp.

−20°C

SMILES string

CCC(C)C1NC(=O)CNC(=O)C2Cc3c([nH]c4cc(O)ccc34)S(=O)CC(NC(=O)CNC1=O)C(=O)NC(CC(N)=O)C(=O)N5CC(O)CC5C(=O)NC(C(C)C(O)CO)C(=O)N2

InChI

1S/C39H54N10O14S/c1-4-16(2)31-36(60)42-11-29(55)43-25-15-64(63)38-21(20-6-5-18(51)7-22(20)46-38)9-23(33(57)41-12-30(56)47-31)44-37(61)32(17(3)27(53)14-50)48-35(59)26-8-19(52)13-49(26)39(62)24(10-28(40)54)45-34(25)58/h5-7,16-17,19,23-27,31-32,46,50-53H,4,8-15H2,1-3H3,(H2,40,54)(H,41,57)(H,42,60)(H,43,55)(H,44,61)(H,45,58)(H,47,56)(H,48,59)

InChI key

CIORWBWIBBPXCG-UHFFFAOYSA-N

Description

Application

α-Amanitin has been used:

for inducing transcriptional arrest in NT2 cells prior to immunofluorescence assay
to induce nephrotoxicity in mice renal tissues
to induce and analyse genotoxicity in mice bone marrow cells by cell viability assay, comet assay and chromosomal aberration assay

Biochem/physiol Actions

Inhibits eukaryotic RNA polymerase II and III; does not inhibit RNA polymerase I or bacterial RNA polymerase; inhibits mammalian protein synthesis.

The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.

Preparation Note

Store a 1 mg/ml aqueous stock solution frozen at -20°C. It does not degrade with repeated freeze-thaw cycles. Dilute into the appropriate buffer immediately prior to use. Product is destroyed by concentrated acid or base.

Disclaimer

When stored frozen in the dark, retained samples were found to be greater than 99% by HPLC after two years.

pictograms

GHS06,GHS08

signalword

Danger

hcodes

H300,H373

Hazard Classifications

Acute Tox. 1 Oral - STOT RE 2

target_organs

Kidney,Blood,Liver,Gastrointestinal tract,Respiratory Tract,Central nervous system

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

pcodes

P260 - P264 - P301 + P310 - P314 - P405 - P501

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

0000526648

0000424280

0000377163

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Global Trade Item Number

SKU	GTIN
A2263-1MG	04061833349946
A2263-100MG	04061833228029