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A2221

Sigma-Aldrich

Antithrombin III from human plasma

lyophilized powder, ≥95% (SDS-PAGE)

Synonym(s):

Factor Xa inhibitor, Heparin cofactor

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About This Item

CAS Number:
90170-80-2
EC Number:
290-516-4
MDL number:
MFCD00166632
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human plasma

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

UniProt accession no.

P01008

storage temp.

−20°C

Gene Information

human ... SERPINC1(462)

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Biochem/physiol Actions

Serine protease inhibitor; Inhibitor of thrombin and activated factor X (Xa). Effective concentration equimolar with proteinase.

Linkage

Not to be confused with thrombin antiserum.

Unit Definition

One unit will form a complex and inhibit one unit of bovine thrombin at 25 degree C, using a 5 minute incubation in the presence of heparin.

Physical form

Lyophilized from 20 mM Tris, pH 8.0

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the specific activity of Antithrombin III from human plasma?

    We do not have strict specifications for the specific activity of the A2221. It is typically in the range of 40-80 units per mg protein. The value is reported on the certificate of analysis for each lot.

  4. What is the molecular weight for Antithrombin III from human plasma. Product A2221?

    The molecular weight of Antithrombin III from human plasma is approximately 53 kDa.

  5. What is Antithrombin III from human plasma, Product A2221, soluble in?

    The manufacturer has found it to be soluble in 50 mM Tris buffer with 150 mM sodium chloride.  (No specific pH or concentration was provided.)

  6. The Antithrombin III from human plasma, Product A2221, vial appears to be empty. What should I do?

    What you are observing is normal for this product. Each vial has sub-milligram amounts in it (either 25 or 125 micrograms), which may not be visible. One should reconstitute the vial, and measure the absorbance at 280 nm to show that it does have protein present.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Alejandro Gugliucci
Life sciences, 82(7-8), 413-418 (2008-01-22)
Conditions in which serum or tissue acrolein levels are high (e.g.: renal failure, heavy smoking, oxidative stress) are also associated with increased thrombogenicity. Another emerging cardiovascular risk factor is homocysteine, and its derivative, homocysteine thiolactone. Antithrombin is one of the
Yu-Fang Hsieh et al.
Biomedical materials (Bristol, England), 12(6), 065004-065004 (2017-10-06)
Biodegradable polymers have potential as a scaffold material for making small diameter artery bypass grafts. To resist thrombosis, maintain biocompatibility and enhance the remodeling of the grafts, it is crucial to modify polymer scaffolds so that the grafts have antithrombogenic
Xiaopeng Tang et al.
Cell research, 30(2), 119-132 (2019-12-08)
Coagulation balance is maintained through fine-tuned interactions among clotting factors, whose physiological concentrations vary substantially. In particular, the concentrations of coagulation proteases (pM to nM) are much lower than their natural inactivator antithrombin (AT, ~ 3 μM), suggesting the existence of other coordinators.
Hao Zhang et al.
Frontiers in cellular and infection microbiology, 11, 677902-677902 (2021-08-03)
Patients with sepsis may progress to acute respiratory distress syndrome (ARDS). Evidence of neutrophil extracellular traps (NETs) in sepsis-induced lung injury has been reported. However, the role of circulating NETs in the progression and thrombotic tendency of sepsis-induced lung injury

Protocols

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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